|
| Status |
Public on Aug 01, 2010 |
| Title |
Plag1 overexpression cooperates with Evi1 overexpression and Gata1s mutation in leading to M7 leukemia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this study is to develop a Plag1 signature and determine how its overexpression contributes to leukemogenesis.
To study this, we transduced an immortalized (but not transformed) cell line (derived from Gata1s mutant fetal liver progenitor through insertional mutagenesis) by Plag1-expressing retrovirus. This turned a non-transformed cell line to a leukemogenic cell line. To study whether Plag1 overexpression led to deregulation of signaling pathways that may contribute to leukemic transformation, we generated microarray gene expression profiles of this cell line transduced with either Plag1 or the empty vector.
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| |
|
| Overall design |
We generated gene expression profiles by microarray from stable cell lines transduced with either the empty vector or the Plag1-expressing vector.
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| |
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| Contributor(s) |
Li Z, Orkin S |
| Citation(s) |
20679399 |
| |
| Submission date |
Jun 17, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Zhe Li |
| E-mail(s) |
li@bloodgroup.tch.harvard.edu
|
| Phone |
617-919-2052
|
| Organization name |
Children's Hospital Boston
|
| Department |
Division of Hematology/Oncology
|
| Lab |
Stuart Orkin's Lab
|
| Street address |
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (8)
|
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| This SubSeries is part of SuperSeries: |
| GSE16655 |
Developmental stage-specific interplay between GATA1 and IGF signaling in fetal hematopoiesis and leukemogenesis |
|
| Relations |
| BioProject |
PRJNA122717 |
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