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| Status |
Public on Apr 01, 2021 |
| Title |
ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics |
| Organism |
Nematostella vectensis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed single-cell transcriptome analysis (using 10x genomics 3' scRNA-seq v3.1 chemistry) in the sea anemone Nematostella vectensis (Cnidaria).
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| Overall design |
Several juvenile polyps were dissociated using ACME method and then single-cells were FACS-sorted before 10x encapsulation and transcriptome capture.
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| Contributor(s) |
Sebe-Pedros A, Iglesias M, Elek A, Garcia-Castro H, Kenny NJ, Solana J |
| Citation(s) |
33827654 |
| |
| Submission date |
Feb 17, 2021 |
| Last update date |
Apr 13, 2021 |
| Contact name |
Arnau Sebe-Pedros |
| E-mail(s) |
arnau.sebe@crg.es
|
| Organization name |
Centre for Genomic Regulation
|
| Department |
Systems Biology
|
| Lab |
Sebe-Pedros lab
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL23802 |
Illumina HiSeq 2500 (Nematostella vectensis) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA702503 |
| SRA |
SRP306882 |
