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Series GSE167019 Query DataSets for GSE167019
Status Public on Nov 04, 2021
Title Chromatin Fiber Folding Represses Transcription and Loop Extrusion in Quiescent Cells (Micro-C XL)
Organism Saccharomyces cerevisiae
Experiment type Other
Summary Determining the conformation of chromatin in cells at the nucleosome level and its relationship to cellular processes has been a central challenge in biology. We show that in quiescent yeast, widespread transcriptional repression coincides with the local compaction of chromatin fibers into structures that are less condensed and more heteromorphic than canonical 30-nanometer forms. Acetylation or substitution of H4 tail residues decompacts fibers and leads to global transcriptional de-repression. Fiber decompaction also increases the rate of loop extrusion by condensin. These findings establish a role for H4 tail-dependent local chromatin fiber folding in regulating transcription and loop extrusion in cells. They also demonstrate the physiological relevance of canonical chromatin fiber folding mechanisms even in the absence of regular 30-nanometer structures.
 
Overall design Examination of log and quiescent cells, including quiescent cells from strains with substitutions in the histone H4 tail basic patch and cells treated with the histone deacetylase inhibitor TSA, by Micro-C XL.
Web link https://elifesciences.org/articles/72062
 
Contributor(s) Swygert SG
Citation(s) 34734806
Submission date Feb 18, 2021
Last update date Nov 06, 2021
Contact name Sarah G. Swygert
E-mail(s) sswygert@fredhutch.org
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences Division
Lab Tsukiyama
Street address 1100 Fairview Avenue North MS A1-162
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (8)
GSM5090893 Log no DSG
GSM5090895 Log
GSM5090897 Q 5toA
This SubSeries is part of SuperSeries:
GSE167020 Chromatin Fiber Folding Represses Transcription and Loop Extrusion in Quiescent Cells
Relations
BioProject PRJNA702747
SRA SRP306975

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE167019_RAW.tar 4.6 Gb (http)(custom) TAR (of HIC)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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