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Status |
Public on Nov 04, 2021 |
Title |
Chromatin Fiber Folding Represses Transcription and Loop Extrusion in Quiescent Cells (Micro-C XL) |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Other
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Summary |
Determining the conformation of chromatin in cells at the nucleosome level and its relationship to cellular processes has been a central challenge in biology. We show that in quiescent yeast, widespread transcriptional repression coincides with the local compaction of chromatin fibers into structures that are less condensed and more heteromorphic than canonical 30-nanometer forms. Acetylation or substitution of H4 tail residues decompacts fibers and leads to global transcriptional de-repression. Fiber decompaction also increases the rate of loop extrusion by condensin. These findings establish a role for H4 tail-dependent local chromatin fiber folding in regulating transcription and loop extrusion in cells. They also demonstrate the physiological relevance of canonical chromatin fiber folding mechanisms even in the absence of regular 30-nanometer structures.
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Overall design |
Examination of log and quiescent cells, including quiescent cells from strains with substitutions in the histone H4 tail basic patch and cells treated with the histone deacetylase inhibitor TSA, by Micro-C XL.
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Web link |
https://elifesciences.org/articles/72062
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Contributor(s) |
Swygert SG |
Citation(s) |
34734806 |
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Submission date |
Feb 18, 2021 |
Last update date |
Nov 06, 2021 |
Contact name |
Sarah G. Swygert |
E-mail(s) |
sswygert@fredhutch.org
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Organization name |
Fred Hutchinson Cancer Research Center
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Department |
Basic Sciences Division
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Lab |
Tsukiyama
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Street address |
1100 Fairview Avenue North MS A1-162
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City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109 |
Country |
USA |
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Platforms (1) |
GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE167020 |
Chromatin Fiber Folding Represses Transcription and Loop Extrusion in Quiescent Cells |
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Relations |
BioProject |
PRJNA702747 |
SRA |
SRP306975 |