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Series GSE167053 Query DataSets for GSE167053
Status Public on Mar 08, 2023
Title Transcriptome analysis of Chd8 S62X heterozygote mutant and wild-type mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Autism spectrum disorders (ASD) are ~4-times more common in males than females, and CHD8 (a chromatin remodeler)-related ASD shows a strong male bias (~5:1), although the underlying mechanism remains unclear. Chd8-mutant mice with a C-terminal protein-truncating mutation (N2373X) display male-preponderant behavioral deficits as juveniles and adults, although whether this also applies to other Chd8 mutations remains unknown. Here we report that new Chd8-mutant mice with a stronger N-terminal protein-truncating mutation (S62X) display male-preponderant autistic-like behaviors at juvenile stages, but as adults, both males and females share behavioral deficits. Excitatory synaptic transmission is suppressed and enhanced in male and female juvenile Chd8+/S62X mice, respectively, but similarly enhanced in adults. ASD-like transcriptomic changes occur monophasically in newborn and juvenile (but not adult) males but biphasically in newborn and adult (not juvenile) females. Therefore, a strong CHD8 mutation induces early-onset ASD-like phenotypes in males but late-onset phenotypes in females after juvenile-stage female protection.
 
Overall design Whole brain transcriptome analysis of Chd8 S62X heterozygote mutant and wild-type mice
 
Contributor(s) Kim E, Lee S, Kweon H, Kang H
Citation(s) 36873104
Submission date Feb 18, 2021
Last update date Mar 08, 2023
Contact name Eunjoon Kim
E-mail(s) kime@kaist.ac.kr
Phone 82-42-350-2633
Organization name IBS/KAIST
Department Center for Synaptic Brain Dysfunctions/Department of Biological Sciences
Street address Kuseong-dong
City Daejeon
ZIP/Postal code 305-701
Country South Korea
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (36)
GSM5091462 P0-M-HT1: P0-male-hetero replicate1
GSM5091463 P0-M-HT2: P0-male-hetero replicate2
GSM5091464 P0-M-HT3: P0-male-hetero replicate3
Relations
BioProject PRJNA702794
SRA SRP307011

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Supplementary file Size Download File type/resource
GSE167053_processed_data_DESeq_count.txt.gz 7.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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