|
| Status |
Public on Mar 01, 2021 |
| Title |
Overexpression of RBFOX1 in HEK293 cells and the effect on circRNA formation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We carried out two mammalian transcriptomics experiments to compare the effect of overexpressing the splicing factor RBFOX1 on circRNA formation. We include quantification of gene expression and circRNA expression.
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| |
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| Overall design |
Each of the experiments used HEK293 cells and comprised six samples, 3 control replicates and 3 RBFOX1 over-expression replicates. Sequencing was performed on both ends of the cDNA fragment (paired-end reads).
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| Contributor(s) |
Gokoolparsadh A, Corley SM, Voineagu I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 23, 2021 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Susan Corley |
| E-mail(s) |
s.corley@unsw.edu.au
|
| Phone |
+61 02 9385 8853
|
| Organization name |
UNSW
|
| Department |
Biotechnology & Biomolecular Sciences
|
| Lab |
Systems Biology Initiative
|
| Street address |
D26, Kensignton Campus
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA704408 |
| SRA |
SRP307800 |
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