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| Status |
Public on Mar 09, 2022 |
| Title |
Effect of in utero nicotine exposure and elastase instillation on transcriptomic profile at post-elastase day 3 in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study is to address if in utero and early life nicotine exposure can affect genetic profile in early ephysema
Methods: A mouse model of two hits: a prenatal hit and a later insult delivered in adulthood was used in this study. In brief, Nicotine (200 mg/L) was supplied immediately after mating and administered to the females in drinking water supplemented with 2% saccharin for the whole period of gestation and until the post-natal day 16 (nicotine group). As a control, we used drinking water with 2% saccharin (control group). Pups born from nicotine-exposed or control mothers were intranasally instilled with elastase or saline at the age of 11 weeks and sacrificed at post-elastase day (ped) 3. Whole lung were collected at ped3 and total RNA was sequenced. We had 4 different groups: control_saline (C-S), control_elastase (C-E), nicotine_saline (N-S) and nicotine_elastase (N-E). ALl the groups had 3 replicas, except N-E group that had 5 different replicas.
Results: At ped3 nicotine upregulated 219 and 2 and downregulated 2 and 109 genes in saline and elastase-instilled samples respectively. Elastase upregulated 723 and 439 and downregulated 116 and 77 genes in controls and nicotine-pretreated samples respectively. Pathway analysis performed with MetaCore software (https://portal.genego.com/) (fold change of 2 or more and p<0.05) revealed many identical pathway activated by elastase instillation in both controls and nicotine-pretreated samples such as the cell cycle, DNA damage and cell adhesion, but also revieled the main difference in activation of the immune system between the controls and nicotine-pretreated samples.
Conclusions: Our results identify the the early nicotine exposure as an important insult that affects the proteomic signature at ped3
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| Overall design |
Lung mRNA profiles of wild type (WT) C57BL/6J mouse lungs were generated using single-end reads with Illumina HiSeq
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| Contributor(s) |
Blaskovic S, Donati Y, Ruchonnet-Metrailler I, Avila Y, Schittny JC, Barazzone-Argiroffo C |
| Citation(s) |
35241086 |
| |
| Submission date |
Feb 25, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Sylvain LEMEILLE |
| Organization name |
University of Geneva Medical School
|
| Department |
Department of Pathology and Immunology
|
| Street address |
1 rue Michel-Servet
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE167569 |
Lung genes modification in elastase-induced emphysema following in utero nicotine exposure |
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| Relations |
| BioProject |
PRJNA704969 |
| SRA |
SRP308246 |
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