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| Status |
Public on Mar 02, 2021 |
| Title |
Accelerated passage of gene-modified monkeys by hormone-induced precocious puberty |
| Organism |
Macaca fascicularis |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
To evaluate the epigenetic status in sperm from the precocious monkeys, whole genome and single-base resolution maps of 5- methylcytosine (mC) analysis was performed by bisulfite-sequencing (BS-Seq) at an average depth of 15.4-fold per strand in DNA sample from the sperm of a naturally-matured monkey (#2) and 13.6-fold from a sexual precocious monkey (#49). Around 70% of all cytosine in genome were covered at least five times in each sample. We analyzed the average methylation levels and density of cytosinein two samples and found that there was no significant difference between the hormone-treated group and control group.
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| |
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| Overall design |
Whole Genome Bisulfite Sequence (WGBS): naturally-matured monkey (#2) and sexual precocious monkey (#49)
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| Contributor(s) |
Sun Q, Liu Z |
| Citation(s) |
34691699 |
| |
| Submission date |
Mar 01, 2021 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Zhen Liu |
| E-mail(s) |
zliu2010@ion.ac.cn
|
| Phone |
15921297531
|
| Organization name |
Institute of Neuroscience (ION), Chinese Academy of Sciences
|
| Department |
Center for Excellence in Brain Science and Intelligence Technology (CEBSIT)
|
| Lab |
Laboratory of Reproductive Engineering in Primates
|
| Street address |
320 Yue-Yang Rd
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
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| Platforms (1) |
| GPL18648 |
Illumina HiSeq 2000 (Macaca fascicularis) |
|
| Samples (2) |
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| Relations |
| BioProject |
PRJNA705598 |
| SRA |
SRP308665 |
