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| Status |
Public on Jun 26, 2009 |
| Title |
Comparative gene expression analysis of 2 subpopulations of dermal papilla cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Different types of hair follicles can be found in the skin of mice. It is believed that the signals that control hair follicle differentiation arise from cells in a structure called the dermal papilla. Understanding the nature of those signals is of interest for the biology of the normal tissue.
We have developed a technique for isolation of dermal cells by enzymatic digestion of intact skin. We have identified two subpopulations of cells that can be separated by FACS. The Sox2-positive CD133-positive cells are found exclusively in the dermal papillae of guard/awl/auchene hairs, while Sox2-negative, CD133-positive cells are found in the other hair follicle types. We compared these populations with unfractionated dermal cells.
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| Overall design |
We isolated the following 3 populations of cells from the back skin of neonatal mice (P2) by Flow Cytometry:
1) GFP-CD133- Total dermal cells
2) GFP-CD133+ Dermal Papilla cells
3) GFP+CD133+ Dermal Papilla cells
The yield is approximately 50,000 cells of each population.
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| |
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| Contributor(s) |
Driskell RR |
| Citation(s) |
19605494 |
| |
| Submission date |
Jun 25, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ryan R Driskell |
| E-mail(s) |
rrd25@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Department of Genetics
|
| Lab |
Watt Lab
|
| Street address |
Tennis Court Rd.
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0NU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
|
| GSM421986 |
GFP-CD133- Cell population (Gate R1) |
| GSM421987 |
GFP-CD133+ Cell Populations (R2) |
| GSM421988 |
GFP+CD133+ Cell Population (Gate R3) #2 |
|
| Relations |
| BioProject |
PRJNA117513 |
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