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Series GSE168084 Query DataSets for GSE168084
Status Public on Jul 06, 2022
Title Sox8 reprograms ectoderm into ear vesicles and associated neurons [Lmx1aE1_rnaseq]
Organism Gallus gallus
Experiment type Expression profiling by high throughput sequencing
Summary The vertebrate inner ear arises from a pool of progenitors with the potential to give rise to all the sense organs and cranial ganglia of the head1-6. Here we explore the molecular mechanisms that control ear specification from these progenitors. Using a multi-omics approach combined with loss-of-function experiments we identify a core transcriptional circuit that imparts ear identity, along with non-coding elements that integrate this information. This analysis places the transcription factor Sox8 at the top of the ear determination network. Introducing Sox8 into cranial ectoderm not only converts non-ear cells into ear progenitors, but also activates the cellular programmes for ear morphogenesis and neurogenesis. Thus, Sox8 emerges as a master regulator of ear identity and may be a key factor for sense organ cell reprogramming.
 
Overall design Chick embryos were electroporated at ss3-6 with either Lmx1aE1- or Sox3U3-EGFP reporter constructs. Embryos were collected at somite stage (ss) 18-21 and processed for cell dissociation. For each sample, 2000 EGFP+ cells were collected by FACS and RNA libraries generated and send for PE 75bp sequencing with HiSeq4000.
 
Contributor(s) Buzzi A, Chen J, Thiery A, Delile J, Streit A
Citation(s) 35867760
Submission date Mar 02, 2021
Last update date Oct 05, 2022
Contact name Andrea Streit
Organization name Kings College London
Department Centre for Craniofacial & Regenerative Biology
Street address Guys Hospital, Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
 
Platforms (1)
GPL23499 Illumina HiSeq 4000 (Gallus gallus)
Samples (8)
GSM5127920 Lmx1aE1_rep1, RNAseq
GSM5127921 Lmx1aE1_rep2, RNAseq
GSM5127922 Lmx1aE1_rep3, RNAseq
This SubSeries is part of SuperSeries:
GSE168089 Sox8 reprogram ectoderm into ear vesicles and auditory neurons
Relations
BioProject PRJNA705997
SRA SRP308879

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Supplementary file Size Download File type/resource
GSE168084_featurecounts.merged.counts.tsv.gz 433.7 Kb (ftp)(http) TSV
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