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| Status |
Public on Jul 06, 2022 |
| Title |
Sox8 reprograms ectoderm into ear vesicles and associated neurons [Lmx1aE1_rnaseq] |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The vertebrate inner ear arises from a pool of progenitors with the potential to give rise to all the sense organs and cranial ganglia of the head1-6. Here we explore the molecular mechanisms that control ear specification from these progenitors. Using a multi-omics approach combined with loss-of-function experiments we identify a core transcriptional circuit that imparts ear identity, along with non-coding elements that integrate this information. This analysis places the transcription factor Sox8 at the top of the ear determination network. Introducing Sox8 into cranial ectoderm not only converts non-ear cells into ear progenitors, but also activates the cellular programmes for ear morphogenesis and neurogenesis. Thus, Sox8 emerges as a master regulator of ear identity and may be a key factor for sense organ cell reprogramming.
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| Overall design |
Chick embryos were electroporated at ss3-6 with either Lmx1aE1- or Sox3U3-EGFP reporter constructs. Embryos were collected at somite stage (ss) 18-21 and processed for cell dissociation. For each sample, 2000 EGFP+ cells were collected by FACS and RNA libraries generated and send for PE 75bp sequencing with HiSeq4000.
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| Contributor(s) |
Buzzi A, Chen J, Thiery A, Delile J, Streit A |
| Citation(s) |
35867760 |
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| Submission date |
Mar 02, 2021 |
| Last update date |
Oct 05, 2022 |
| Contact name |
Andrea Streit |
| Organization name |
Kings College London
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| Department |
Centre for Craniofacial & Regenerative Biology
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| Street address |
Guys Hospital, Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL23499 |
Illumina HiSeq 4000 (Gallus gallus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE168089 |
Sox8 reprogram ectoderm into ear vesicles and auditory neurons |
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| Relations |
| BioProject |
PRJNA705997 |
| SRA |
SRP308879 |
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