 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 06, 2021 |
| Title |
Translational activation by an alternative sigma factor in Bacillus subtilis |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Other
|
| Summary |
Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, B, in Bacillus subtilis paradoxically leads to dramatic induction of translation for a subset of its regulon genes. These genes are translationally repressed when transcribed by the housekeeping sigma factor, A, owing to extended RNA secondary structures as determined in vivo using DMS-MaPseq. Transcription fromB-dependent promoters liberates the secondary structures and activates translation, leading to dual induction. Translation efficiencies between B- and A-dependent RNA isoforms can vary by up to 100-fold, which in multiple cases exceeds the magnitude of transcriptional induction. These results highlight the role of long-range RNA folding in modulating translation and demonstrate that a transcription factor can regulate protein synthesis beyond its effects on transcript levels.
|
| |
|
| Overall design |
RNA was isolated from BS168 cells treated with DMS in vivo and from untreated cells. Regions of the σA-dependent isoforms of ctc and yvrE were specifically reverse-transcribed from each sample using TGIRT-III, which encoded DMS-induced RNA modifications as mutations in the resulting DNA amplicons. Libraries were prepared for sequencing following the established DMS-MaPseq protocol from Tomezsko et al., Nature, 2020 and Zubradt et al., Nature Methods, 2016. DMS signals were quantified using the DREEM algorithm from Tomezsko et al., Nature, 2020 and used as folding constraints to determine the RNA structure for each isoform.
|
| |
|
| Contributor(s) |
McCormick DM, Lalanne J, Lan TC, Rouskin S, Li G |
| Citation(s) |
33927010 |
| |
| Submission date |
Mar 06, 2021 |
| Last update date |
Jun 08, 2021 |
| Contact name |
Gene-Wei Li |
| E-mail(s) |
gwli@mit.edu
|
| Phone |
617-324-6703
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Gene-Wei Li Lab
|
| Street address |
31 Ames Street, 68-223A
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23473 |
Illumina MiSeq (Bacillus subtilis subsp. subtilis str. 168) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA707207 |
| SRA |
SRP309584 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE168393_RAW.tar |
20.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...