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| Status |
Public on Sep 14, 2021 |
| Title |
Homoeologous Chromosome Exchange Explains the Creation of a QTL Affecting Soil‐Borne Pathogen Resistance in Tobacco |
| Organism |
Nicotiana tabacum |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Investigate genes associated with Phn7.1, a major QTL influencing partial resistance to the soil-borne pathogen Phytophthora nicotianae in tobacco.
Methods: Resistant and susceptible tobacco near isogenic lines with and without Phn7.1 QTL were subjected to the inoculation with Phytophthora nicotianae suspension and suspension buffer without pathogen as control followed by sample collection at 42 hour past inoculation for RNA-seq analysis.
Results: Revealed gene expression profiles associated disease resistance and susceptiblilty.
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| |
|
| Overall design |
Tobacco Hicks near isogenic lines with and without Phn7.1 QTL were inoculated with Phytophthora nicotianae race 0 suspension and suspension buffer without pathogen as control. The root treated 42 hours post inoculation were used for RNA purificaton and RNAseq sequencing.
|
| Web link |
http://doi: 10.1111/pbi.13693
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| |
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| Contributor(s) |
Lewis R, Shi R |
| Citation(s) |
34453871 |
| |
| Submission date |
Mar 14, 2021 |
| Last update date |
Sep 15, 2021 |
| Contact name |
Ramsey Lewis |
| E-mail(s) |
rslewis@ncsu.edu
|
| Phone |
(919)-513-4802
|
| Organization name |
North Carolina State University
|
| Department |
Crop and Soil Sciences Department
|
| Lab |
Tobacco Breeding & Genetics
|
| Street address |
101 Derieux PI
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25653 |
Illumina HiSeq 4000 (Nicotiana tabacum) |
|
| Samples (14)
|
| GSM5171509 |
root, PC1 |
| GSM5171510 |
root, PC2 |
| GSM5171511 |
root, PC3 |
| GSM5171512 |
root, HI1 |
| GSM5171513 |
root, HI2 |
| GSM5171514 |
root, HI3 |
| GSM5171515 |
root, PI1 |
| GSM5171516 |
root, PI2 |
| GSM5171517 |
root, PI3 |
| GSM5171518 |
root, PI4 |
| GSM5171519 |
root, PI5 |
|
| Relations |
| BioProject |
PRJNA714375 |
| SRA |
SRP310543 |
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