GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE169356 Query DataSets for GSE169356
Status Public on Apr 01, 2022
Title Integrated omics profiling reveals FOXA1 and Ku70/Ku80 as the direct target of ivermectin for prostate carcinogenic inhibition
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Here, we show that ivermectin suppress prostate cancer progression by inhibiting AR signaling pathway and attenuate cellular DNA damage repair capacity. We applied an integrated omics profiling including RNA-seq and Thermal proteome, that found pioneer factor Forkhead Box Protein A1 (FOXA1) and Non-homologous End Joining (NHEJ) repair executer Ku70/Ku80 was the direct target of Ivermectin in prostate cancer. Ivermectin binds to these two proteins and block their biological function, which results in blockade of AR signaling transcription and deficiency of DNA double-strand breaks (DSBs) repair system, and thereby leads to G0/G1 arrest and trigger synthetic lethality Our findings demonstrate both the effect and target of Ivermectin in prostate cancer comprehensively and systemically, indicating that use of Ivermectin may constitute a new therapeutic approach for prostate cancer.
Overall design C4-2 and 22RV1 cells were treated with 8 or 12 μM ivermectin for 48 hours and the transcription profiling was analyzed by RNA-seq
Contributor(s) Lv S, Wu Z, Wang Z, Wei Q
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 22, 2021
Last update date Apr 01, 2022
Contact name Shidong Lv
Phone +8615622178285
Organization name Nanfang Hospital, Southern Medical University
Department Urology
Street address Guangzhou Ave. North No. 1838
City Guangzhou
ZIP/Postal code 510515
Country China
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (18)
GSM5196899 C4-2_DMSO_rep1
GSM5196900 C4-2_DMSO_rep2
GSM5196901 C4-2_DMSO_rep3
BioProject PRJNA716321
SRA SRP311687

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE169356_22RV1_raw_gene_counts_matrix.txt.gz 546.3 Kb (ftp)(http) TXT
GSE169356_C4-2_raw_gene_counts_matrix.txt.gz 530.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap