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| Status |
Public on Apr 01, 2022 |
| Title |
Integrated omics profiling reveals FOXA1 and Ku70/Ku80 as the direct target of ivermectin for prostate carcinogenic inhibition |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we show that ivermectin suppress prostate cancer progression by inhibiting AR signaling pathway and attenuate cellular DNA damage repair capacity. We applied an integrated omics profiling including RNA-seq and Thermal proteome, that found pioneer factor Forkhead Box Protein A1 (FOXA1) and Non-homologous End Joining (NHEJ) repair executer Ku70/Ku80 was the direct target of Ivermectin in prostate cancer. Ivermectin binds to these two proteins and block their biological function, which results in blockade of AR signaling transcription and deficiency of DNA double-strand breaks (DSBs) repair system, and thereby leads to G0/G1 arrest and trigger synthetic lethality Our findings demonstrate both the effect and target of Ivermectin in prostate cancer comprehensively and systemically, indicating that use of Ivermectin may constitute a new therapeutic approach for prostate cancer.
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| Overall design |
C4-2 and 22RV1 cells were treated with 8 or 12 μM ivermectin for 48 hours and the transcription profiling was analyzed by RNA-seq
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| Contributor(s) |
Lv S, Wu Z, Wang Z, Wei Q |
| Citation missing |
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| |
| Submission date |
Mar 22, 2021 |
| Last update date |
Apr 01, 2022 |
| Contact name |
Shidong Lv |
| E-mail(s) |
lsd990@gmail.com
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| Phone |
+8615622178285
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| Organization name |
Nanfang Hospital, Southern Medical University
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| Department |
Urology
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| Street address |
Guangzhou Ave. North No. 1838
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| City |
Guangzhou |
| ZIP/Postal code |
510515 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA716321 |
| SRA |
SRP311687 |
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