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| Status |
Public on May 11, 2022 |
| Title |
Transcriptome analysis of miniBacillus PG10 and its parental strain B. subtilis 168 |
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
miniBacillus PG10 lacks ~36% of dispensible genentic material and has been derived from B. subtilis 168 (Reuß et al. 2017; PMID: 27965289). We performed RNA-seq analysis on mid-to-late exponential cultures and stationary phase cultures that were grown in LB medium. We specifically analyzed differential gene expression levels of lipid metabolic genes to compare to the lipidomic profiles of the two strains.
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| Overall design |
12 samples; 3 replicates
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| Contributor(s) |
van Tilburg AY, Warmer P, van Heel AJ, Sauer U, P Kuipers O |
| Citation(s) |
34856064 |
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| Submission date |
Mar 23, 2021 |
| Last update date |
May 12, 2022 |
| Contact name |
Anne de Jong |
| E-mail(s) |
anne.de.jong@rug.nl
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| Phone |
+31 50 363 2047
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| Organization name |
university of Groningen
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| Department |
Molecular Genetics
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| Street address |
Nijenborgh 7
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| City |
Groningen |
| ZIP/Postal code |
9747 AG |
| Country |
Netherlands |
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| Platforms (1) |
| GPL21796 |
Illumina NextSeq 500 (Bacillus subtilis subsp. subtilis str. 168) |
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| Samples (12)
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| GSM5202057 |
B. subtilis 168, exponential phase, rep. #1 |
| GSM5202058 |
B. subtilis 168, exponential phase, rep. #2 |
| GSM5202059 |
B. subtilis 168, exponential phase, rep. #3 |
| GSM5202060 |
B. subtilis 168, stationary phase, rep. #1 |
| GSM5202061 |
B. subtilis 168, stationary phase, rep. #2 |
| GSM5202062 |
B. subtilis 168, stationary phase, rep. #3 |
| GSM5202063 |
PG10, exponential phase, rep. #1 |
| GSM5202064 |
PG10, exponential phase, rep. #2 |
| GSM5202065 |
PG10, exponential phase, rep. #3 |
| GSM5202066 |
PG10, stationary phase, rep. #1 |
| GSM5202067 |
PG10, stationary phase, rep. #2 |
| GSM5202068 |
PG10, stationary phase, rep. #3 |
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| Relations |
| BioProject |
PRJNA716536 |
| SRA |
SRP311787 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE169409_RAW.tar |
100.5 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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