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| Status |
Public on Nov 03, 2009 |
| Title |
Time course of the mRNA expression after bacteriophage ϕYS40 infection in wild-type Thermus thermophilus HB8 strain. |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain during infection of bacteriophage ϕYS40.
Keywords: time course, bacteriophage, infection, wild type
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| Overall design |
Three wild-type T. thermophilus HB8 strains were pre-cultured at 70°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C until A600 value being ~0.8 (1.7 × 108 cells/ml) that corresponds to logarithmic growth phase. Then the ΦYS40 phage was infected to the medium at multiplicity of infection (m.o.i.) being ~1, and continued cultivation. Cells were collected after 0, 25, 50, 75 and 100 min infection, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.
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| Contributor(s) |
Agari Y, Sakamoto K, Osaki A, Ohmori M, Kuramitsu S, Shinkai A |
| Citation(s) |
19891975 |
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| Submission date |
Jul 06, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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| URL |
http://www.srg.harima.riken.jp/
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| Organization name |
RIKEN Harima Institute
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| Department |
SPring-8 Center
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| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL4902 |
Riken Thermus thermophilus HB8 4K mRNA array |
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| Samples (15)
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| GSM424658 |
wild-type strain on a rich medium, phage ϕYS40 pre-infection (1) |
| GSM424659 |
wild-type strain on a rich medium, phage ϕYS40 pre-infection (2) |
| GSM424660 |
wild-type strain on a rich medium, phage ϕYS40 pre-infection (3) |
| GSM424662 |
wild-type strain on a rich medium, 25 (min) time point after phage ϕYS40 infection (1) |
| GSM424665 |
wild-type strain on a rich medium, 25 (min) time point after phage ϕYS40 infection (2) |
| GSM424666 |
wild-type strain on a rich medium, 25 (min) time point after phage ϕYS40 infection (3) |
| GSM424667 |
wild-type strain on a rich medium, 50 (min) time point after phage ϕYS40 infection (1) |
| GSM424668 |
wild-type strain on a rich medium, 50 (min) time point after phage ϕYS40 infection (2) |
| GSM424669 |
wild-type strain on a rich medium, 50 (min) time point after phage ϕYS40 infection (3) |
| GSM424670 |
wild-type strain on a rich medium, 75 (min) time point after phage ϕYS40 infection (1) |
| GSM424671 |
wild-type strain on a rich medium, 75 (min) time point after phage ϕYS40 infection (2) |
| GSM424672 |
wild-type strain on a rich medium, 75 (min) time point after phage ϕYS40 infection (3) |
| GSM424673 |
wild-type strain on a rich medium, 100 (min) time point after phage ϕYS40 infection (1) |
| GSM424674 |
wild-type strain on a rich medium, 100 (min) time point after phage ϕYS40 infection (2) |
| GSM424675 |
wild-type strain on a rich medium, 100 (min) time point after phage ϕYS40 infection (3) |
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| This SubSeries is part of SuperSeries: |
| GSE16978 |
Time course of bacteriophage ϕYS40 infection in wild-type and crp deletion Thermus thermophilus HB8 strain |
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| Relations |
| BioProject |
PRJNA122673 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16954_RAW.tar |
26.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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