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| Status |
Public on Mar 26, 2022 |
| Title |
Nutrient sensing mediated by O-GlcNAcylation regulates gene expression through epigenetic mechanisms during embryonic neurogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We show that pharmacological upregulation of O-GlcNAc levels during hESCs-neuronal differentiation leads to upregulation of key neurogenic TF gene which in turn is associated with increased H3K4me3 levels, reduction in H3K27me3 levels and increase in Pol IISer5 phosphorylation. Our results demonstrate O-GlcNAc mediated sensitivity of chromatin to nutrient status, and indicate how metabolic dysregulations could affect stem cell fate decisions through epigenetic gene regulatory mechanisms
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| Overall design |
RNA-seq in four stages of hESC neural differentiation on samples treated with TMG and solvent control
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| Contributor(s) |
Ansari SA, Parween S |
| Citation(s) |
35470239 |
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| Submission date |
Mar 25, 2021 |
| Last update date |
May 11, 2022 |
| Contact name |
Priyankara J Wickramasinghe |
| E-mail(s) |
priyaw@wistar.org
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| Phone |
2154956837
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| Organization name |
The Wistar Institute
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| Department |
Bioinformatics
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| Lab |
Genomics
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| Street address |
3601 Spruce Street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (16)
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| GSM5211739 |
D0 TMG, replicate 2 |
| GSM5211740 |
D12 control, replicate 1 |
| GSM5211741 |
D12 control, replicate 2 |
| GSM5211742 |
D12 TMG, replicate 1 |
| GSM5211743 |
D12 TMG, replicate 2 |
| GSM5211744 |
D30 control, replicate 1 |
| GSM5211745 |
D30 control, replicate 2 |
| GSM5211746 |
D30 TMG, replicate 1 |
| GSM5211747 |
D30 TMG, replicate 2 |
| GSM5211748 |
D70 control, replicate 1 |
| GSM5211749 |
D70 control, replicate 2 |
| GSM5211750 |
D70 TMG, replicate 1 |
| GSM5211751 |
D70 TMG, replicate 2 |
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| Relations |
| BioProject |
PRJNA717264 |
| SRA |
SRP312176 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE169649_RNAseq_counts.txt.gz |
875.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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