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| Status |
Public on Jul 30, 2021 |
| Title |
Connection of core and and tail Mediator modules restrains transcription from TFIID-dependent promoters [ChEC-seq] |
| Organism |
Saccharomyces cerevisiae W303 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The Mediator coactivator complex is divided into four modules: head, middle, tail, and kinase. Deletion of the architectural subunit Med16 separates core Mediator (cMed), comprising the head, middle, and scaffold (Med14), from the tail. However, the direct global effects of tail/cMed disconnection are unclear. We find that rapid depletion of Med16 downregulates genes that require the SAGA complex for full expression, consistent with their reported tail dependence, but also moderately overactivates TFIID-dependent genes in a manner partly dependent on the separated tail, which remains associated with upstream activating sequences. Suppression of TBP dynamics via removal of the Mot1 ATPase partially restores normal transcriptional activity to Med16-depleted cells, suggesting that cMed/tail separation results in an imbalance in the levels PIC formation at SAGA-requiring and TFIID-dependent genes. We suggest that the preferential regulation of SAGA-requiring genes by tailed Mediator helps maintain a proper balance of transcription between these genes and those more dependent on TFIID.
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| Overall design |
ChEC-seq of Mediator subunits in Med16 deletion or depletion strains.
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| Contributor(s) |
Saleh MM, Zentner GE |
| Citation(s) |
34383744 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM128631 |
Regulation of transcription by the Mediator complex |
INDIANA UNIVERSITY |
GABRIEL ZENTNER |
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| Submission date |
Mar 26, 2021 |
| Last update date |
Aug 17, 2021 |
| Contact name |
Gabriel E Zentner |
| E-mail(s) |
gzentner@indiana.edu
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| Phone |
812-856-7377
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| Organization name |
Indiana University
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| Department |
Biology
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| Street address |
915 E 3rd St
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| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
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| Platforms (1) |
| GPL27477 |
Illumina NextSeq 500 (Saccharomyces cerevisiae W303) |
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| Samples (67)
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| GSM5213389 |
Free-MNase in WT, rep-1 |
| GSM5213390 |
Free-MNase in WT, rep-2 |
| GSM5213391 |
Free-MNase in WT, rep-3 |
| GSM5213392 |
MED13 in med16-deletion, rep-1 |
| GSM5213393 |
MED13 in med16-deletion, rep-2 |
| GSM5213394 |
MED13 in med16-deletion, rep-3 |
| GSM5213395 |
MED13 in WT, rep-1.1 |
| GSM5213396 |
MED13 in WT, rep-2.1 |
| GSM5213397 |
MED13 in WT, rep-3.1 |
| GSM5213398 |
MED14 in med16-deletion, rep-1 |
| GSM5213399 |
MED14 in med16-deletion, rep-2 |
| GSM5213400 |
MED14 in med16-deletion, rep-3 |
| GSM5213401 |
MED14 in WT, rep-2 |
| GSM5213402 |
MED14 in WT, rep-3 |
| GSM5213403 |
MED15 in med16-deletion, rep-1 |
| GSM5213404 |
MED15 in med16-deletion, rep-2 |
| GSM5213405 |
MED15 in med16-deletion, rep-3 |
| GSM5213406 |
MED15 in WT, rep-1 |
| GSM5213407 |
MED15 in WT, rep-2 |
| GSM5213408 |
MED15 in WT, rep-3 |
| GSM5213409 |
MED16 in WT, rep-1 |
| GSM5213410 |
MED16 in WT, rep-2 |
| GSM5213411 |
MED16 in WT, rep-3 |
| GSM5213412 |
MED2 in med16-deletion, rep-1 |
| GSM5213413 |
MED2 in med16-deletion, rep-2 |
| GSM5213414 |
MED2 in med16-deletion, rep-3 |
| GSM5213415 |
MED2 in WT, rep-1 |
| GSM5213416 |
MED2 in WT, rep-2 |
| GSM5213417 |
MED2 in WT, rep-3 |
| GSM5213418 |
MED3 in med16-deletion, rep-1 |
| GSM5213419 |
MED3 in med16-deletion, rep-2 |
| GSM5213420 |
MED3 in med16-deletion, rep-3 |
| GSM5213421 |
MED3 in WT, rep-2 |
| GSM5213422 |
MED3 in WT, rep-3 |
| GSM5213423 |
MED5 in med16-deletion, rep-1 |
| GSM5213424 |
MED5 in med16-deletion, rep-2 |
| GSM5213425 |
MED5 in med16-deletion, rep-3 |
| GSM5213426 |
MED5 in WT, rep-1 |
| GSM5213427 |
MED5 in WT, rep-2 |
| GSM5213428 |
MED5 in WT, rep-3 |
| GSM5213429 |
MED8 in med16-deletion, rep-1 |
| GSM5213430 |
MED8 in med16-deletion, rep-2 |
| GSM5213431 |
MED8 in med16-deletion, rep-3 |
| GSM5213432 |
MED8 in med16-deletion, rep-4 |
| GSM5213433 |
MED8 in WT, rep-1 |
| GSM5213434 |
MED8 in WT, rep-3 |
| GSM5213435 |
MED9 in med16-deletion, rep-1 |
| GSM5213436 |
MED9 in med16-deletion, rep-2 |
| GSM5213437 |
MED9 in med16-deletion, rep-3 |
| GSM5213438 |
MED9 in WT, rep-1 |
| GSM5213439 |
MED9 in WT, rep-2 |
| GSM5213440 |
MED9 in WT, rep-3 |
| GSM5213441 |
Med5-ChEC in Med16-AID, +DMSO, rep-1 |
| GSM5213442 |
Med5-ChEC in Med16-AID, +IAA, rep-1 |
| GSM5213443 |
Med14-ChEC in Med16-AID, +DMSO, rep-1 |
| GSM5213444 |
Med14-ChEC in Med16-AID, +IAA, rep-1 |
| GSM5213445 |
Med14-ChEC in Med16-AID, +IAA, rep-2 |
| GSM5213446 |
Med2-ChEC in Med16-AID, +DMSO, rep-1 |
| GSM5213447 |
Med2-ChEC in Med16-AID, +DMSO, rep-2 |
| GSM5213448 |
Med2-ChEC in Med16-AID, +IAA, rep-1 |
| GSM5213449 |
Med2-ChEC in Med16-AID, +IAA, rep-2 |
| GSM5213450 |
Med5-ChEC in Med16-AID, +DMSO, rep-2 |
| GSM5213451 |
Med14-ChEC in Med16-AID, +DMSO, rep-2 |
| GSM5213452 |
Med5-ChEC in Med16-AID, +IAA, rep-2 |
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| This SubSeries is part of SuperSeries: |
| GSE169748 |
Connection of core and and tail Mediator modules restrains transcription from TFIID-dependent promoters |
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| Relations |
| BioProject |
PRJNA717792 |
| SRA |
SRP312362 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE169746_RAW.tar |
783.6 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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