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| Status |
Public on Mar 25, 2022 |
| Title |
Transcriptomics study of excess Mn (1000 uM) in Chlamydomonas reinhardtii |
| Organism |
Chlamydomonas reinhardtii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Liquid cultures of the unicellular green alga, Chlamydomonas reinhardtii were grown in media with 6 uM Mn (control) or 1000 uM Mn (experimental), and analyzed by RNA-Seq to identify genes that are differentially expressed in response to excess Mn.
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| Overall design |
Six flasks containing Tris-Acetate-Phosphorus (TAP) medium were innoculated with C. reinhardtii from a starter culture. Three of the flasks contained 6 uM Mn and three flasks contained 1000 uM Mn. These were grown on a shaker platform under continuous light until they reached a cell density of 3x10^6 cells/mL (mid-log). Total RNA was purified from each culture and subjected to RNA-Seq analysis on the Illumina HiSeq2000 platform.
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| Contributor(s) |
Gallaher SD, Tsednee M, Castruita M, Merchant SS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 26, 2021 |
| Last update date |
Mar 25, 2022 |
| Contact name |
Sean D. Gallaher |
| Organization name |
UC Berkeley
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| Department |
California Institute for Quantitative Biosciences
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| Lab |
Sabeeha Merchant
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| Street address |
176 Stanley Hall, room 408
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| City |
Berkeley |
| State/province |
California |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL15922 |
Illumina HiSeq 2000 (Chlamydomonas reinhardtii) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA717804 |
| SRA |
SRP312374 |
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