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| Status |
Public on Apr 30, 2012 |
| Title |
Gene expression in mature pollen and sperm cells versus young seedling as a vegetative sporophyte reference control |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Sperm cells represent the male partner that fuses with the egg cell during fertilization in all multi-cellular eukaryotic organisms, and, in flowering plants, is a founder of both embryo and nutritive endosperm. We examined the transcriptome of Oryza sativa ssp. japonica using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated in the paternal gamete.
We used microarrays to detail the global program of gene expression in mature pollen and sperm cells at anthesis, which constitutes the stage immediately preceding pollen germination and gamete deposition leading up to fertilization. This documents the condition of the male gametophytic cells prior to fertilization.
Keywords: tissue expression profile, male gametophyte, sperm, pollen, seedling
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| Overall design |
Male gametophytic cells consisting of isolated pollen and isolated sperm cells collected at anthesis were compared with seedlings containing leaf, stem, root and apical meristems as a vegetative sporophytic control; three separate biological replicates were used.
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| Contributor(s) |
Russell SD, Gou X |
| Citation(s) |
19825578, 21892935, 22716952, 21731498 |
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| Submission date |
Jul 08, 2009 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Scott D Russell |
| E-mail(s) |
srussell@ou.edu
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| Phone |
405 210-8935
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| Organization name |
University of Oklahoma
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| Department |
Dept of Microbiology and Plant Biology
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| Street address |
770 Van Vleet Oval
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| City |
Norman |
| State/province |
OK |
| ZIP/Postal code |
73019 |
| Country |
USA |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (9)
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| GSM425463 |
Seedling, V3 stage, biological rep 1 |
| GSM425464 |
Seedling, V3 stage, biological rep 2 |
| GSM425465 |
Seedling, V3 stage, biological rep 3 |
| GSM425466 |
Pollen at anthesis, biological rep 1 |
| GSM425467 |
Pollen at anthesis, biological rep 2 |
| GSM425468 |
Pollen at anthesis, biological rep 3 |
| GSM425469 |
Sperm at anthesis, biological rep 1 |
| GSM425470 |
Sperm at anthesis, biological rep 2 |
| GSM425471 |
Sperm at anthesis, biological rep 3 |
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| Relations |
| BioProject |
PRJNA117739 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17002_RAW.tar |
40.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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