 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 11, 2009 |
| Title |
Variation in the Genomic islands of porcine enteropathogenic E. coli strains of serogroup O45 revealed by CGH and PCR. |
| Platform organism |
Escherichia coli |
| Sample organisms |
Escherichia coli; Escherichia coli O157:H7; Escherichia coli O157:H7 str. EDL933; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Genome variation profiling by array
|
| Summary |
Background: Members of E. coli serogroup O45 are porcine enteropathogenic E. coli (PEPEC) strains which cause post-weaning diarrhea and produce characteristic attaching and effacing (A/E) lesions. Most of O45 PEPEC strains possess the locus of enterocyte effacement (LEE), encoding the virulence factors for A/E lesions, and often possess the paa gene, which is thought to contribute to the early stages of PEPEC virulence.
Methodology: Nine O45 PEPEC strains and a rabbit enteropathogenic (REPEC) strain, known to produce A/E lesions, were characterized using an E. coli O157-E. coli K12 whole genome microarray and a virulence gene-specific microarray, and by PCR experiments.
Results: Based on their virulence genes profiles, the 10 strains were characterized as atypical EPEC. The differences in their genomes pointed to two distinct evolutionary groups of O45 PEPEC, Group I and Group II, and to the contribution these genetic differences have on virulence in pigs. Group I contained the REPEC strain and four O45 PEPEC strains known to induce severe A/E lesions in challenged pigs whereas Group II was composed of five other O45 PEPEC strains which induced less severe or no A/E lesions in challenged pigs. Significant differences between Groups I and II were found in the presence or absence of 50 O-Islands (OIs) or S-loops and 13 K-islands (KIs) or K-loops, including the virulence-associated islands OI#1 (S-loop#1), OI#47 (S-loop#71), OI#57 (S-loop#85), OI#71 (S-loop#108), OI#115, OI#122, and OI#154 (S-loop#253).
|
| |
|
| Overall design |
10 samples, with two microarrays per sample. Each microarray includes duplicates of every spot.
|
| |
|
| Contributor(s) |
Bruant G, Zhang Y, Garneau P, Wong J, Laing C, Fairbrother JM, Gannon VP, Harel J |
| Citation(s) |
19709428 |
| |
| Submission date |
Jul 10, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Chad R Laing |
| E-mail(s) |
chad_r_laing@phac-aspc.gc.ca
|
| Organization name |
Public Health Agency of Canada
|
| Department |
Laboratory for Foodborne Zoonoses
|
| Street address |
Twp Rd 9-1
|
| City |
Lethbridge |
| State/province |
Alberta |
| ZIP/Postal code |
T1J3Z4 |
| Country |
Canada |
| |
|
| Platforms (1) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA117835 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17036_RAW.tar |
26.6 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...