|
| Status |
Public on Jul 17, 2009 |
| Title |
Pseudomonas aeruginosa PA14, mvfR and anthranilic acid analogs |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
|
| Summary |
The Pseudomonas aeruginosa MvfR-dependent QS regulatory pathway controls the expression of key virulence genes; and is activated via the extracellular signals 4-hydroxy-2-heptylquinoline (HHQ) and 3,4-dihydroxy-2-heptylquinoline (PQS), whose syntheses depend on anthranilic acid (AA), the primary precursor of 4-hydroxy-2-alkylquinolines (HAQs). We identified halogenated AA analogs that specifically inhibited HAQ biosynthesis and disrupted MvfR-dependent gene expression. These compounds restricted P. aeruginosa systemic dissemination and mortality in mice, without perturbing bacterial viability, and inhibited osmoprotection, a widespread bacterial function.
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| Overall design |
mvfR mutants of PA14 and control PA14 cells were grown to OD 2.5, a point where QS is turned on, and their expression profiles were compared.
AA analogs 4CABA and 6CABA were added directly to the culture media of PA14 cells.
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| |
|
| Contributor(s) |
Rahme L, Deziel E, Lesic B, Lepine F, Xiao G, He J |
| Citation(s) |
17941706, 19497948, 15686549, 20300606 |
| Submission date |
Jul 15, 2009 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Laurence G Rahme |
| E-mail(s) |
rahme@molbio.mgh.harvard.edu
|
| Phone |
617-724-5003
|
| Fax |
617-724-8558
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Surgery
|
| Street address |
50 Blosson St, Thier 340
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
|
| Samples (10)
|
|
| This SubSeries is part of SuperSeries: |
| GSE17147 |
Pseudomonas aeruginosa PA14 MvfR-dependent QS regulatory pathway |
|
| Relations |
| BioProject |
PRJNA123453 |
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