|
| Status |
Public on Apr 12, 2021 |
| Title |
Gastruloids generated without exogenous Wnt activation develop anterior neural tissues [EmbryoidsTruseq2] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we describe an approach to generate gastruloids that have a more complete antero-posterior development. We used bioengineered hydrogel microwell arrays to promote the robust derivation of mouse ESCs into post-implantation epiblast-like (EPI) aggregates in a reproducible and scalable manner. These EPI aggregates break symmetry and axially elongate without external chemical stimulation. Inhibition of WNT signaling in early stages of development leads to the formation of gastruloids with anterior neural tissues.
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| |
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| Overall design |
Testing the effect of XAV939 on the development of mouse epiblast-like aggregates.
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| |
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| Contributor(s) |
Girgin MU, Broguiere N, Mattolini L, Lutolf MP |
| Citation(s) |
33891872 |
| |
| Submission date |
Mar 31, 2021 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Nicolas Broguiere |
| E-mail(s) |
nicolas.broguiere@epfl.ch
|
| Organization name |
EPFL
|
| Department |
Sciences de la vie
|
| Lab |
Laboratory of Stem Cell Bioengineering
|
| Street address |
AI 1208 Station 15
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA718749 |
| SRA |
SRP312864 |
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