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Status |
Public on Jul 16, 2010 |
Title |
NP-18 vs. NP-18AR transcriptional profile upon treatment with TK/GCV |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Pancreatic cancer-derived cells NP-18 underwent four rounds of treatment with increasing doses of an adenoviral vector encoding TK enzyme and GCV. Surviving cells were termed NP-18AR and displayed decreased sensitivity to treatment. This experiment analyses the transcriptomic effect of TK/GCV treatment on NP-18AR as compared to that of NP-18 cells.
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Overall design |
Two-condition experiment, where response to treatment in naïve cells (NP-18) was compared to response to treatment in previously treated resistant cells (NP-18AR). Biological replicates: samples from 3 independently generated and independently treated NP-18AR lines (NP-18AR1, NP-18AR2 and NP-18AR3) were hibridized with 3 independently treated samples of NP-18 cells. A total of 9 hybridizations were performed, 2 for NP-18AR1 (including 1 dye-swap), 4 for NP-18AR2 (including 2 dye swaps) and 3 for NP-18AR3 (including 1 dye-swap).
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Contributor(s) |
Abate-Daga D, Sumoy L, Fillat C |
Citation(s) |
20599444 |
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Submission date |
Jul 16, 2009 |
Last update date |
Dec 06, 2012 |
Contact name |
Lauro Sumoy |
E-mail(s) |
lsumoy@igtp.cat
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Organization name |
IGTP
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Department |
High Content Genomics and Bioinformatics
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Street address |
Ctra. Can Ruti, Camí de les escoles s/n
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City |
Badalona |
State/province |
Barcelona |
ZIP/Postal code |
08916 |
Country |
Spain |
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Platforms (1) |
GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
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Samples (9)
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GSM428999 |
Resistant versus Sensitive replicate 1 |
GSM429000 |
Resistant versus Sensitive replicate 1 dye swap |
GSM429001 |
Resistant versus Sensitive replicate 2 |
GSM429002 |
Resistant versus Sensitive replicate 2 dye swap |
GSM429003 |
Resistant versus Sensitive replicate 2 bis |
GSM429004 |
Resistant versus Sensitive replicate 2 bis dye swap |
GSM429005 |
Resistant versus Sensitive replicate 3 |
GSM429006 |
Resistant versus Sensitive replicate 3 dye swap |
GSM429007 |
Resistant versus Sensitive replicate 3 bis |
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Relations |
BioProject |
PRJNA119817 |