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| Status |
Public on Jul 16, 2010 |
| Title |
NP-18 vs. NP-18AR transcriptional profile upon treatment with TK/GCV |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Pancreatic cancer-derived cells NP-18 underwent four rounds of treatment with increasing doses of an adenoviral vector encoding TK enzyme and GCV. Surviving cells were termed NP-18AR and displayed decreased sensitivity to treatment. This experiment analyses the transcriptomic effect of TK/GCV treatment on NP-18AR as compared to that of NP-18 cells.
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| Overall design |
Two-condition experiment, where response to treatment in naïve cells (NP-18) was compared to response to treatment in previously treated resistant cells (NP-18AR). Biological replicates: samples from 3 independently generated and independently treated NP-18AR lines (NP-18AR1, NP-18AR2 and NP-18AR3) were hibridized with 3 independently treated samples of NP-18 cells. A total of 9 hybridizations were performed, 2 for NP-18AR1 (including 1 dye-swap), 4 for NP-18AR2 (including 2 dye swaps) and 3 for NP-18AR3 (including 1 dye-swap).
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| Contributor(s) |
Abate-Daga D, Sumoy L, Fillat C |
| Citation(s) |
20599444 |
| |
| Submission date |
Jul 16, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Lauro Sumoy |
| E-mail(s) |
lsumoy@igtp.cat
|
| Organization name |
IGTP
|
| Department |
High Content Genomics and Bioinformatics
|
| Street address |
Ctra. Can Ruti, Camí de les escoles s/n
|
| City |
Badalona |
| State/province |
Barcelona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
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| Samples (9)
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| GSM428999 |
Resistant versus Sensitive replicate 1 |
| GSM429000 |
Resistant versus Sensitive replicate 1 dye swap |
| GSM429001 |
Resistant versus Sensitive replicate 2 |
| GSM429002 |
Resistant versus Sensitive replicate 2 dye swap |
| GSM429003 |
Resistant versus Sensitive replicate 2 bis |
| GSM429004 |
Resistant versus Sensitive replicate 2 bis dye swap |
| GSM429005 |
Resistant versus Sensitive replicate 3 |
| GSM429006 |
Resistant versus Sensitive replicate 3 dye swap |
| GSM429007 |
Resistant versus Sensitive replicate 3 bis |
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| Relations |
| BioProject |
PRJNA119817 |
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