|
| Status |
Public on Nov 23, 2022 |
| Title |
ΔNp63/p73 drive metastatic colonization by controlling a regenerative epithelial stem cell program in quasi-mesenchymal cancer stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
p63/p73 are master transcriptional regulators of ITGB4-hi cancer stem cells and are required to maintain CSCs in a quasi-mesenchymal state and for metastatic colonization
|
| |
|
| Overall design |
single-cell RNA-seq of tdTom+ carcinoma cells isolated from the lungs of NSG mice 3 days after tail-vein injection. ITGB4-hi control (NT) or p63/p73 knockout (dKO) cells were injected into mice (n=5). Three days later lungs was dissected, dissociated, and pooled together for each group. tdTom+ carcinoma cells were isolated by flow cytometry and immediated processed for single-cell RNA-seq.
|
| Web link |
https://www.cell.com/developmental-cell/fulltext/S1534-5807(22)00815-2
|
| |
|
| Contributor(s) |
Lamber A, Yuan B, Gupta S, Weinberg R |
| Citation(s) |
36538894 |
| |
| Submission date |
Apr 07, 2021 |
| Last update date |
Mar 10, 2023 |
| Contact name |
Arthur W. Lambert |
| Organization name |
Whitehead Institute
|
| Lab |
Robert A. Weinberg
|
| Street address |
455 Main street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE172407 |
p63/p73 maintain cancer stem cells in a quasi-mesenchymal state and drive metastatic colonization via EGFR signaling |
|
| Relations |
| BioProject |
PRJNA720335 |
| SRA |
SRP313801 |
