|
| Status |
Public on Jul 30, 2021 |
| Title |
Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters [ChIP-Seq] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The Mediator coactivator complex is divided into four modules: head, middle, tail, and kinase. Deletion of the architectural subunit Med16 separates core Mediator (cMed), comprising the head, middle, and scaffold (Med14), from the tail. However, the direct global effects of tail/cMed disconnection are unclear. We find that rapid depletion of Med16 downregulates genes that require the SAGA complex for full expression, consistent with their reported tail dependence, but also moderately overactivates TFIID-dependent genes in a manner partly dependent on the separated tail, which remains associated with upstream activating sequences. Suppression of TBP dynamics via removal of the Mot1 ATPase partially restores normal transcriptional activity to Med16-depleted cells, suggesting that cMed/tail separation results in an imbalance in the levels of PIC formation at SAGA-requiring and TFIID-dependent genes. We suggest that the preferential regulation of SAGA-requiring genes by tailed Mediator helps maintain a proper balance of transcription between these genes and those more dependent on TFIID.
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| |
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| Overall design |
ChIP-seq of Mediator in Wildtype and Med16-deletion
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| |
|
| Contributor(s) |
Saleh MM, Zentner GE, Jeronimo C, Robert F |
| Citation(s) |
34383744 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM128631 |
Regulation of transcription by the Mediator complex |
INDIANA UNIVERSITY |
GABRIEL ZENTNER |
|
| |
| Submission date |
Apr 13, 2021 |
| Last update date |
Oct 29, 2021 |
| Contact name |
Gabriel E Zentner |
| E-mail(s) |
gzentner@indiana.edu
|
| Phone |
812-856-7377
|
| Organization name |
Indiana University
|
| Department |
Biology
|
| Street address |
915 E 3rd St
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
|
| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE169748 |
Connection of core and and tail Mediator modules restrains transcription from TFIID-dependent promoters |
|
| Relations |
| BioProject |
PRJNA721611 |
| SRA |
SRP314639 |
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