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| Status |
Public on Dec 14, 2021 |
| Title |
Regulation of translation under mitoprotein-induced stress in Saccharomyces cerevisiae |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
Proteotoxic stress triggers adaptive cellular responses, including changes in gene expression on the levels of transcription and translation. In this study, we analyzed the translational response of yeast cells to impaired protein import into mitochondria, a condition under which mitochondrial precursor proteins accumulate in the cytosol and impose proteotoxic stress. We analyzed changes in translational efficiency as well as more subtle changes in the distribution of ribosomes along transcripts, with a special focus on translation initiation sites.
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| Overall design |
Ribosome footprints from clogger-expressing and control cells after 4.5 h of clogger induction were isolated and sequenced in 3 biological replicates. Cells in replicate 1 were treated with cycloheximide before cell lysis to enrich translation initiation sites. Replicates 2 and 3 were not in contact with cycloheximide before cell lysis.
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| Contributor(s) |
Boos F, Stein KC, Frydman J |
| Citation(s) |
34786732 |
| Submission date |
Apr 13, 2021 |
| Last update date |
Dec 16, 2021 |
| Contact name |
Felix Christian Boos |
| E-mail(s) |
fboos@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Genetics
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| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
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| Samples (6)
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| GSM5239802 |
cyt-1: cyt DHFR - with CHX - Replicate 1 |
| GSM5239803 |
cyt-2: cyt DHFR - without CHX - Replicate 2 |
| GSM5239804 |
cyt-3: cyt DHFR - without CHX - Replicate 3 |
| GSM5239805 |
b2-1: b2-DHFR - with CHX - Replicate 1 |
| GSM5239806 |
b2-2: b2-DHFR - without CHX - Replicate 2 |
| GSM5239807 |
b2-3: b2-DHFR - without CHX - Replicate 3 |
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| Relations |
| BioProject |
PRJNA721705 |
| SRA |
SRP314712 |
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