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| Status |
Public on Sep 07, 2022 |
| Title |
The Skeletal Muscle Molecular Clock Regulates Sarcomere Length Through Titin Splicing [1] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Circadian rhythms have been implicated in regulating skeletal muscle structure and function, but no mechanisms have connected the molecular clock to sarcomeric proteins. We identified an isoform shift in the sarcomeric ruler, titin, and showed that the skeletal muscle molecular clock regulates titin isoform and subsequently sarcomere length through RBM20, an RNA binding protein that controls titin splicing.
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| Overall design |
Tibialis anterior muscles were collected from inducible, skeletal muscle specific Bmal1 fl/fl mice 5 weeks after reatment with either vehicle or tamoxifen solution. Muscles were pulverized under liquid nitrogen to prevent fiber type bias prior to total RNA colleciton.
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| Contributor(s) |
Riley LA, Esser KA |
| Citation(s) |
36047761 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 AR070625 |
The molecular clock and titin expression in skeletal muscle |
UNIVERSITY OF FLORIDA |
Lance Riley |
| R01 AR066082 |
Molecular clock and skeletal muscle weakness |
UNIVERSITY OF FLORIDA |
Karyn A Esser |
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| |
| Submission date |
Apr 14, 2021 |
| Last update date |
Sep 07, 2022 |
| Contact name |
Lance Riley |
| E-mail(s) |
lance.riley.91@gmail.com
|
| Organization name |
Vanderbilt University
|
| Department |
Biomedical Engineering
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| Lab |
Merryman Mechanobiology Lab
|
| Street address |
9445 MRB4, 2213 Garland Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE189865 |
The Skeletal Muscle Molecular Clock Regulates Sarcomere Length Through Titin Splicing |
|
| Relations |
| BioProject |
PRJNA721950 |
| SRA |
SRP314826 |
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