|
| Status |
Public on Apr 17, 2021 |
| Title |
miRNA array to determine changes in sEV miRNA cargo composition in endothelial cells during inflammatory response |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
We performed miRNA micro array to asses the miRNA profile differences in sEVs during inflammation vs. homeostatic conditions in endothelial cells. Therefore HUVEC cells were treated with a polytrauma cocktail comprised of IL1ß, IL6, CXCL8, C3a and C5a-des-Arg (PTC) at concentrations measured in patient serum 24hrs after polytrauma
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| Overall design |
Confluent HUVEC cells were treated with PTC in serum-free medium for 24hrs before supernatant was harvested and sEVs purified. Total RNA was then extracted from corresponding sEVs and used for miRNA microarray to analyze miRNA composition
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| Contributor(s) |
Seufferlein T, Eiseler T, Seibold T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 16, 2021 |
| Last update date |
Apr 18, 2021 |
| Contact name |
Tim Eiseler |
| E-mail(s) |
tim.eiseler@uniklinik-ulm.de
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| Organization name |
Ulm University
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| Department |
Internal Medicine 1
|
| Street address |
Albert-Einstein Allee 23
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| City |
Ulm |
| ZIP/Postal code |
89081 |
| Country |
Germany |
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| Platforms (1) |
| GPL21575 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (Feature Number version) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE172212 |
Small extracellular vesicles propagate the inflammatory response after trauma. |
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| Relations |
| BioProject |
PRJNA722439 |
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