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| Status |
Public on Jan 10, 2022 |
| Title |
Single cell RNAseq of mouse colon stroma during chronic DSS and homeostatic condition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We implemented a murine model of intestinal inflammation based on oral administration of multiple cycles of low dose dextran sulfate sodium (DSS) to induce epithelial injury and ECM deposition. Mice were subjected to 3 repetitive cycles of DSS displayed progressive accumulation of immune cell infiltrates associated with excessive deposition of collagen fibers. Lamina propria cells from water-fed and DSS-fed mice were isolated using enzymatic digestion, and enriched for stromal cells by FACS using antibodies excluding hematopoietic cells (CD45), epithelial cells (EpCAM), and erythrocytes. Prepared single cell suspensions were then profiled using the 10x Chromium V2 droplet-based single cell RNA sequencing platform. Tissues were collected from proximal and distal colon.
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| Overall design |
A total of 3 female mice (12 weeks old) were processed for each treatment group and analyzed by single-cell RNA-seq. Female mice were utilized because they were able to withstand 3 cycles of DSS, whereas males exhibited morbidity.
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| Contributor(s) |
Jasso G, Jaiswal A, Graham D, Xavier R |
| Citation(s) |
35085231 |
| |
| Submission date |
Apr 16, 2021 |
| Last update date |
Apr 11, 2022 |
| Contact name |
Alok Jaiswal |
| E-mail(s) |
ajaiswal@broadinstitute.org
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| Organization name |
The Broad Institute of MIT and Harvard
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| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MASSACHUSETTS |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA722540 |
| SRA |
SRP315153 |
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