Transcriptional profiling of human genital skin biopsies at lesion, 2- and 8-weeks post healing and contra-lateral control genital skin biopsies during recurrent HSV-2 infection
Analysis of host response at the gene expression level during lesion and post healing periods (2- & 8-weeks after HSV-2 reactivation induced lesion is resolved). We would like to get a comprehensive overview on host gene expression of genital skin during lesion and post healing periods of recurrent HSV-2 infection as compared to those in control skin.
Overall design
Total RNA were extracted from tissue slices of genital skin biopsies isolated from patients with recurrent HSV-2 infection using PicoPure kits (Thermo Scientific). cDNA were amlified and biotin labled using NuGEN kit, and hybridized to Illumina beadarrays (HT-12 v4).
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