|
| Status |
Public on Apr 21, 2021 |
| Title |
Gene expression profiling of Trypanosoma cruzi in the presence of heme points to glycosomal metabolic adaptation of epimastigotes inside the vector |
| Organism |
Trypanosoma cruzi |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using the Deep Seq transcriptome sequencing we characterized the heme induced transcriptome of epimastigotes and determined that most of the upregulated genes were related to glucose metabolism inside the glycosomes.
|
| |
|
| Overall design |
the RNA-Seq analysis should be a valuable screening tool strategy for identifying stage-regulated T. cruzi genes and metabolic pathways affected by heme
|
| |
|
| Contributor(s) |
Paes MC, Alcocer M, Sang F |
| Citation(s) |
31895927 |
| |
| Submission date |
Apr 20, 2021 |
| Last update date |
Apr 21, 2021 |
| Contact name |
Fei Sang |
| E-mail(s) |
mbzfs1@exmail.nottingham.ac.uk
|
| Organization name |
University of Nottingham
|
| Department |
School of Life Sciences
|
| Lab |
DeepSeq
|
| Street address |
QMC
|
| City |
Nottingham |
| ZIP/Postal code |
NG72UH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL27740 |
Illumina NextSeq 500 (Trypanosoma cruzi) |
|
| Samples (5)
|
| GSM5257024 |
f2_S14: epimastigotes rep1, control |
| GSM5257025 |
f3_S1: epimastigotes rep2, control |
| GSM5257026 |
H1_S9: epimastigotes rep1, supplemented with haemoglobin |
| GSM5257027 |
H2_S3: epimastigotes rep2, supplemented with haemoglobin |
| GSM5257028 |
H3_S5: rep3, epimastigotes supplemented with haemoglobin |
|
| Relations |
| BioProject |
PRJNA723332 |
| SRA |
SRP315589 |
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