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| Status |
Public on Nov 10, 2021 |
| Title |
RNA-sequencing of human pluripotent stem-cell derived endothelial cells under control and Wnt-activating conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Endothelial cells (ECs) in the central nervous system (CNS) acquire specialized barrier properties in response to extrinsic signals, with Wnt/β-catenin signaling coordinating multiple aspects of endothelial barrier function. We used human pluripotent stem cell (hPSC)-derived endothelial progenitor cells to profile the EC response to Wnt activation using multiple strategies, including the small molecule GSK-3 inhibitor CHIR 99021, and the CNS-derived ligands Wnt7a and Wnt7b.
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| Overall design |
20 total samples were analyzed, 18 endothelial cell (EC) samples and 2 smooth muscle-like cell (SMLC) samples. All cells were differentiated from the human induced pluripotent stem cell line iPS(IMR90)-4. Four independent differentiations were performed (denoted 2, 3, 5, and 6). At Passage 1, ECs treated with DMSO (vehicle control), 4 uM CHIR 99021 (CHIR), or 50 ng/ml each Wnt7a and Wnt7b (WNT) were analyzed from differentiations 2, 3, 5, and 6. At Passage 3, ECs treated with DMSO or CHIR were analyzed from differentiations 2, 3, and 6. At Passage 1, SMLCs treated with DMSO were analyzed from differentiations 3 and 6. For Passage 1 samples, fluoresence-activated cell sorting was used to isolate CD31+ (EC) and CD31- (SMLC) populations.
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| Contributor(s) |
Gastfriend BD, Palecek SP, Shusta EV |
| Citation(s) |
34755601 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS103844 |
Investigating Pericyte Roles in Blood-Brain Barrier Formation |
UNIVERSITY OF WISCONSIN MADISON |
ERIC V SHUSTA |
| R01 NS107461 |
Mechanisms of Shear Induction of Blood-Brain Barrier Phenotypes in Human iPSC-derived Brain Endothelial Progenitors |
UNIVERSITY OF WISCONSIN MADISON |
Sean P Palecek |
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| Submission date |
Apr 23, 2021 |
| Last update date |
Nov 11, 2021 |
| Contact name |
Benjamin D Gastfriend |
| Organization name |
University of Wisconsin-Madison
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| Street address |
1415 Engineering Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| GSM5261814 |
P1DMSO2: Passage 1 DMSO-treated endothelial cells 2 |
| GSM5261815 |
P1DMSO3: Passage 1 DMSO-treated endothelial cells 3 |
| GSM5261816 |
P1DMSO5: Passage 1 DMSO-treated endothelial cells 5 |
| GSM5261817 |
P1DMSO6: Passage 1 DMSO-treated endothelial cells 6 |
| GSM5261818 |
P1CHIR2: Passage 1 CHIR-treated endothelial cells 2 |
| GSM5261819 |
P1CHIR3: Passage 1 CHIR-treated endothelial cells 3 |
| GSM5261820 |
P1CHIR5: Passage 1 CHIR-treated endothelial cells 5 |
| GSM5261821 |
P1CHIR6: Passage 1 CHIR-treated endothelial cells 6 |
| GSM5261822 |
P1WNT2: Passage 1 Wnt7a/b-treated endothelial cells 2 |
| GSM5261823 |
P1WNT3: Passage 1 Wnt7a/b-treated endothelial cells 3 |
| GSM5261824 |
P1WNT5: Passage 1 Wnt7a/b-treated endothelial cells 5 |
| GSM5261825 |
P1WNT6: Passage 1 Wnt7a/b-treated endothelial cells 6 |
| GSM5261826 |
P3DMSO2: Passage 3 DMSO-treated endothelial cells 2 |
| GSM5261827 |
P3DMSO3: Passage 3 DMSO-treated endothelial cells 3 |
| GSM5261828 |
P3DMSO6: Passage 3 DMSO-treated endothelial cells 6 |
| GSM5261829 |
P3CHIR2: Passage 3 CHIR-treated endothelial cells 2 |
| GSM5261830 |
P3CHIR3: Passage 3 CHIR-treated endothelial cells 3 |
| GSM5261831 |
P3CHIR6: Passage 3 CHIR-treated endothelial cells 6 |
| GSM5261832 |
P1SMLC3: Passage 1 DMSO-treated smooth muscle-like cells 3 |
| GSM5261833 |
P1SMLC6: Passage 1 DMSO-treated smooth muscle-like cells 6 |
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| Relations |
| BioProject |
PRJNA724690 |
| SRA |
SRP316053 |
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