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| Status |
Public on Sep 03, 2021 |
| Title |
Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models |
| Organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single nuclei sequencing of grafts developed 14 weeks post transplantation of human embryonic stem cell derived pancreatic progenitors alone (PP) or with rat adipose derived microvessels (PPMV) into the subcutaneous pocket of diabetic (STZ-induced) Scid-beige mice.
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| Overall design |
- hESC H1 line was differentiated to stage 4 pancreatic progenitors and embedded in collagen hydrogels alone (PP) or with microvessel fragments (PPMV) freshly isolated from epididymal fat of Sprague-Dawley rats.
- The PP and PPMV containing hydrogels were transplanted subcutaneously into STZ-induced diabetic Scid-beige male mice.
- 14 weeks post-transplantation the grafts were retrieved from 3 mice, transplanted with independent PP differentiations and 3 mice transplanted with matching PP and independent MV isolations.
- The 3 corresponding grafts were merged to generate 1 PP and 1 PPMV sample.
- The two samples were subjected to nuclei isolation and single nuclei sequencing.
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| Contributor(s) |
Aghazadeh Y, Poon F, Sarangi F, Wong FT, Khan ST, Sun X, Hatkar R, Cox BJ, Nunes SS, Nostro MC |
| Citation(s) |
34480863 |
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| Submission date |
Apr 23, 2021 |
| Last update date |
Dec 05, 2021 |
| Contact name |
Yasaman Aghazadeh |
| E-mail(s) |
yassaman.aghazadeh@gmail.com
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| Organization name |
McEwen Stem Cell Institute
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| Lab |
Nostro lab
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| Street address |
101, College St
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (2) |
| GPL25431 |
Illumina HiSeq 4000 (Homo sapiens; Mus musculus) |
| GPL30035 |
Illumina HiSeq 4000 (Homo sapiens; Mus musculus; Rattus norvegicus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA724727 |
| SRA |
SRP316099 |
