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| Status |
Public on Jul 01, 2015 |
| Title |
miRNA expression of 3 prostate cell types from human normal donors |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Prostate primary epithelial and stromal cells were isolated from normal donorhuman prostate tissue and patient prostate tumors (>Gleason 8) using in vitroselective media conditions and differential substrate adhesion properties.Tumor cells specific to the prostate epithelial cell component attached tomatrigel while epithelial cells from donors preferentially attached to gelatinsubstrate. The prostate tumor cells did not proliferate over time in culturebut cells of normal epithelial phenotype in comparison to the donor tissueeventually emerged from this cell compartment. The epithelial componentof both donor and tumor specimens produced stem cell colonies thatexpressed OCT4 in growth factor reduced media but subsequently formedembryoid bodies in hanging drop cultures which differentiated into multiplegerm line lineages under the influence of growth factors. To determineif prostate tumor-derived stem cells were capable of forming tumors invivo, we compared the behavior of the donor and tumor stem cells aftertransplantation as tissue recombinants under the renal capsule of SCID micein the presence of rat urogenital mesenchyme (rUGM). Stem cells from bothdonor and tumor sources produced glandular structures that secreted prostatespecific antigen (PSA) while no glandular structures were formed from tissue recombinants of normal epithelial or tumor epithelial cells plus rUGM or fromrUGM alone. Serial transplantation of the stem cell recombinants from tumor specimens yielded subrenal structures reflecting an abundance of glandswith morphological features typical of Prostatic Intraepithelial Neoplasia(PIN). In order to determine whether intrinsic differences existed amongthe donor-derived and tumor-derived stem cells, array based microRNAexpression profiling was performed on all cell types obtained after in vitro isolation.
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| Overall design |
Sample Labeling Key:
PC-Prostate Cancer
PD-Prostate Donor (Normal Tissue)
DNE-Donor Normal Epithelial
NS-Donor Normal Stem
TNE-Tumor Normal Epithelial
TE-Tumor Epithelial
(TNE and TE samples with corresponding numbers are matched pairs)
TS-Tumor Stem Cell
S-Stromal
PC 73 TNE was run in all 6 batches as a control for batch effect. The number in parentheses corresponds with the batch the sample was run in.
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| Contributor(s) |
LaFramboise WA, Bacich D, Petrosko P, Krill-Burger JM, Sciulli C, Dhir R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 24, 2009 |
| Last update date |
Jul 01, 2015 |
| Contact name |
John Michael Krill-Burger |
| E-mail(s) |
burgerm@upmc.edu
|
| Phone |
412-656-6727
|
| Organization name |
University of Pittsburgh Medical Center
|
| Street address |
Rm. WG21.3 Shadyside Hospital
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
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| Platforms (1) |
| GPL7724 |
miRCURY LNA microRNA Array, v. 9.2, all organisms |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA119001 |
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