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| Status |
Public on Apr 30, 2023 |
| Title |
RNA-Seq data for murine BM Tx transduced with different sgRNA combinations [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To elucidate the mutational interplay of Ezh2, Runx1 and Bcor we transplanted mice transplanted with LSKs transduced with sgRNAs in all combinations and observed them for development of myeloid leukemia and subsequent expression analysis of whole bone marrow by RNA-Seq.
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| Overall design |
LSK cells expressing SpCas9 were transduced with sgRNA combinations and transplanted into irradiated C57Bl/6J mice. BM was harvested after development of a leukemia or after 6 months and used for RNA extraction.
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| Contributor(s) |
Borchert D, Heckl D |
| Citation missing |
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| |
| Submission date |
Apr 28, 2021 |
| Last update date |
Apr 30, 2023 |
| Contact name |
Dorit Borchert |
| E-mail(s) |
Borchert.Dorit@mh-hannover.de
|
| Organization name |
Hannover Medical School
|
| Department |
Pediatric Hematology and Oncology
|
| Street address |
Carl-Neuberg-Str. 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE173497 |
Oncofetal gene reactivation drives EZH2-mutant myeloid leukemia pathogenesis |
|
| Relations |
| BioProject |
PRJNA725839 |
| SRA |
SRP316736 |
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