GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE173496 Query DataSets for GSE173496
Status Public on Apr 30, 2023
Title RNA-Seq data for murine BM Tx transduced with different sgRNA combinations [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To elucidate the mutational interplay of Ezh2, Runx1 and Bcor we transplanted mice transplanted with LSKs transduced with sgRNAs in all combinations and observed them for development of myeloid leukemia and subsequent expression analysis of whole bone marrow by RNA-Seq.
Overall design LSK cells expressing SpCas9 were transduced with sgRNA combinations and transplanted into irradiated C57Bl/6J mice. BM was harvested after development of a leukemia or after 6 months and used for RNA extraction.
Contributor(s) Borchert D, Heckl D
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 28, 2021
Last update date Apr 30, 2023
Contact name Dorit Borchert
Organization name Hannover Medical School
Department Pediatric Hematology and Oncology
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (22)
GSM5268648 sgLuc rpl1
GSM5268649 sgLuc rpl2
GSM5268650 sgLuc rpl3
This SubSeries is part of SuperSeries:
GSE173497 Oncofetal gene reactivation drives EZH2-mutant myeloid leukemia pathogenesis
BioProject PRJNA725839
SRA SRP316736

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE173496_RNA_Seq_genes.FPKM.annot.xlsx 10.6 Mb (ftp)(http) XLSX
GSE173496_RNA_Seq_genes.readcount.annot.xlsx 5.9 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap