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Series GSE173509 Query DataSets for GSE173509
Status Public on Apr 29, 2021
Title Time-course transcriptomic profiling of cell-cycle entry in MCF10A cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To identify potential novel regulators of G1/S transition and S-phase progression, we performed an RNA sequencing (RNA-Seq) time-course analysis of non-transformed human breast epithelial cells (MCF10A) released into the cell cycle from serum starvation, in the presence of DMSO or a specific CDK4/6 inhibitor palbociclib.
 
Overall design Conditions include: 0hr, 3hr DMSO, 6hr DMSO, 9hr DMSO, 9hr palbociclib, 12hr DMSO, 12hr palbociclib, 15hr DMSO, 15hr palbociclib, 24hr DMSO, 24hr palbociclib. A total of 31 samples containing triplicates (except 24-hour time point, which was performed in duplicates) were analyzed.
 
Contributor(s) Fan Y, Köberlin MS, Liu C, Meyer T
Citation(s)
  • Fan Y, Köberlin MS, Ratnayeke N, Liu C et al. LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol 2021 Aug 2;220(8). PMID: 34037657
Submission date Apr 28, 2021
Last update date Jul 29, 2021
Contact name Tobias Meyer
Organization name Stanford University
Street address 318 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (31)
GSM5268960 MCF10A replicate1-0h
GSM5268961 MCF10A replicate1-3h DMSO
GSM5268962 MCF10A replicate1-6h DMSO
Relations
BioProject PRJNA725870
SRA SRP316754

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE173509_GEO_raw_counts.txt.gz 1.4 Mb (ftp)(http) TXT
GSE173509_RAW.tar 138.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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