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GEO help: Mouse over screen elements for information. |
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Status |
Public on Apr 29, 2021 |
Title |
Time-course transcriptomic profiling of cell-cycle entry in MCF10A cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To identify potential novel regulators of G1/S transition and S-phase progression, we performed an RNA sequencing (RNA-Seq) time-course analysis of non-transformed human breast epithelial cells (MCF10A) released into the cell cycle from serum starvation, in the presence of DMSO or a specific CDK4/6 inhibitor palbociclib.
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Overall design |
Conditions include: 0hr, 3hr DMSO, 6hr DMSO, 9hr DMSO, 9hr palbociclib, 12hr DMSO, 12hr palbociclib, 15hr DMSO, 15hr palbociclib, 24hr DMSO, 24hr palbociclib. A total of 31 samples containing triplicates (except 24-hour time point, which was performed in duplicates) were analyzed.
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Contributor(s) |
Fan Y, Köberlin MS, Liu C, Meyer T |
Citation(s) |
- Fan Y, Köberlin MS, Ratnayeke N, Liu C et al. LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol 2021 Aug 2;220(8). PMID: 34037657
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Submission date |
Apr 28, 2021 |
Last update date |
Jul 29, 2021 |
Contact name |
Tobias Meyer |
Organization name |
Stanford University
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Street address |
318 Campus Dr
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City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (31)
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Relations |
BioProject |
PRJNA725870 |
SRA |
SRP316754 |
Supplementary file |
Size |
Download |
File type/resource |
GSE173509_GEO_raw_counts.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
GSE173509_RAW.tar |
138.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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