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| Status |
Public on Dec 31, 2009 |
| Title |
Regulatory T cell-mediated resolution of lung injury: Identification of potential target genes via expression profiling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We examined early and late gene expression changes using the IT LPS model of Acute Lung Injury (ALI). In this model, injury peaks at day 4 and is almost completely resolved by day 10 in wild type (WT) C57BL/6 mice. In contrast to the pattern in WT mice, lymphocyte-deficient Rag-1 -/- mice exhibit strikingly delayed resolution despite similar initial injury.
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| Overall design |
Total RNA was isolated from mouse lung at time 0, 1, 4, and 10 days following LPS treatment of wild type and Rag-1 null mice using TRIzol reagent (Invitrogen Life Technologies), then purified using RNAeasy columns (Qiagen) with DNase I treatment according to the manufacturer’s recommendations. The quality of RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies).
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| Contributor(s) |
Grigoryev DN, Aggarwal NR |
| Citation(s) |
20028937 |
| |
| Submission date |
Jul 27, 2009 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Dmitry N Grigoryev |
| E-mail(s) |
dgrigor1@jhmi.edu
|
| Phone |
410 550 1557
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| Organization name |
JHU
|
| Lab |
HopGene
|
| Street address |
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL4865 |
Sentrix MouseRef-8 Expression BeadChip (Target ID) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA119073 |
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