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| Status |
Public on May 21, 2022 |
| Title |
Airway administration of flagellin regulates the inflammatory response to Pseudomonas aeruginosa |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here, we aim to understand the role of P38 in the airway epithelial cells response to flagellin. We report the gene expression profile of pig airway epithelial cells cultured under air-liquid conditions, pre-incubated or not with the P38 inhibitor SB203580 (20 µM, Tocris Bioscience) for 1 h and then stimulated with 100 ng/ml of a mutated flagellin (FliCΔ174-400) for 2h or 24h. Our data show that flagellin stimulation induce the expression of pro-inflammatory cytokines mainly through the NFkB pathway, with little impact of P38 inhibition in the cells response.
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| Overall design |
RNA-seq analysis of airway epithelial cells pre-incubated (SB) or not (DMSO) with the P38 inhibitor SB203580 and stimulated with 100 ng/ml of flagellin for 2 or 24h.
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| Contributor(s) |
Caballero I |
| Citation missing |
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| |
| Submission date |
May 03, 2021 |
| Last update date |
May 21, 2022 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
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| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
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| Street address |
660 route des lucioles
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| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
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| Platforms (1) |
| GPL20983 |
Illumina NextSeq 500 (Sus scrofa) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA726987 |
| SRA |
SRP318238 |
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