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Series GSE17395 Query DataSets for GSE17395
Status Public on Dec 31, 2009
Title A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by genome tiling array
Summary Cwc21 (complexed with CEF1 protein 21) is a 135 amino acid yeast protein that shares homology with the N-terminal domain of human SRm300, a large serine/arginine-repeat protein shown previously to associate with the splicing coactivator SRm160. Proteomic analysis of spliceosomal complexes has suggested a role for Cwc21 and SRm300 at the core of the spliceosome. However, specific functions for these proteins have remained elusive. In this report we employ quantitative genetic interaction mapping, mass-spectrometry of tandem affinity purified complexes and microarray profiling to investigate genetic, physical and functional interactions involving Cwc21. Combined data from these assays support multiple roles for Cwc21 in the formation and function of splicing complexes. Consistent with a role for Cwc21 at the core of the spliceosome, we observe strong genetic, physical and functional interactions with Isy1, a protein previously implicated in the first catalytic step of splicing and splicing fidelity. Together, the results suggest multiple functions for Cwc21/SRm300 in the splicing process, including an important role in the activation of splicing in association with Isy1.
Overall design S. cerevisiae mutants with single- and double-deletions for cwc21 and isy1 or cwc21 and bud13 were grown under standard conditions and genome-wide expression profiles were obtained using Affymetrix tiling microarrays. Pre-mRNA splicing defects were then assessed in pairwise comparisons with wild-type strains that were grown and assayed under the same conditions.
Contributor(s) Khanna M, van Bakel H, Tang Y, Calarco JA, Babak T, Guo G, Emili A, Greenblatt JF, Hughes TR, Krogan NJ, Blencowe BJ
Citation(s) 19789211
Submission date Jul 29, 2009
Last update date Mar 21, 2012
Contact name Harm van Bakel
Organization name Mount Sinai School of Medicine
Department Genetics and Genomic Sciences
Lab Bakel Lab
Street address One Gustave L. Levy Place, Box 1498
City New York
State/province New York
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL7070 Scerevisiae_tlg Array
Samples (5)
GSM434585 cwc21-null expression profile
GSM434586 isy1-null expression profile
GSM434587 bud13-null expression profile
BioProject PRJNA118835

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17395_RAW.tar 422.8 Mb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file

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