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| Status |
Public on Nov 22, 2021 |
| Title |
Growth phase-associated changes in the proteome and transcriptome of Bifidobacterium animalis subsp. lactis A6 |
| Organism |
Bifidobacterium animalis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study was aimed to further illustrate the expression files of BAA6 between the stationary phase and the exponential phase in MRSc broth, we combined the transcriptomic analysis with RNA sequencing and tandem mass tags (TMT) quantitative proteomic methods to quantitatively screen the differentially expressed genes and proteins. During the stationary phase, BAA6 expanded its carbon source utilizing profile and enhanced the transport of cellobiose, xylooligosaccharides (XOS), and raffinose to confront with glucose consumption. Meanwhile, the increased NH3 production, enhanced branched-chain amino acids (BCAAs) transport and biosynthesis, and upregulated GroEL/ES complex and Clp protease could contribute to respond to acid stress during fermentation. These findings provide a novel insight into the growth characteristics developed by B. animalis to environment changes.
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| Overall design |
Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations
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| Contributor(s) |
Wang H, Hao Y |
| Citation(s) |
35209838 |
| |
| Submission date |
May 05, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Hui Wang |
| E-mail(s) |
wanghui1201@cau.edu.cn
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| Organization name |
China Agricultural University
|
| Street address |
No. 17 Tsing Hua East Road, HaiDian District
|
| City |
Beijing |
| ZIP/Postal code |
100085 |
| Country |
China |
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| Platforms (1) |
| GPL30086 |
Illumina HiSeq 2500 (Bifidobacterium animalis) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA727606 |
| SRA |
SRP318634 |
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