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| Status |
Public on Jun 06, 2023 |
| Title |
Bulk RNA sequencing from sorted thymic populations and tumors from control Cdc20(+/D) and aneuploid Cdc20(D/AAA)mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to compare the transcriptome profile of specific thymic populations in control Cdc20(+/D) and Cdc20(D/AAA)mice after the induction of chromosome missegregation as well the profile of tumors generated in those mice.
Methods: Total RNA was isolated from primary cells or tumors, using the RNeasy Plus Mini Kit (Qiagen) and sequenced on an Illumina HiSeq2000. At least 5000 cells were sorted directly in lysis buffer for specifc T cell progenitor populations in the thymus while at least 5 million cells have used for RNA isolation form thymic lymphomas. Unless otherwise indicated samples from tumors were isolated without sorting specific polulations.
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| Overall design |
bulk RNA-seq of tumor and non-tumor thymus of control Cdc20(+/D) and Cdc20(D/AAA) mice.
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| Contributor(s) |
Trakala M, Amon A |
| Citation(s) |
34266888 |
| Submission date |
May 06, 2021 |
| Last update date |
Jun 06, 2023 |
| Contact name |
Dunaduan Ma |
| E-mail(s) |
maduanduan8@gmail.com
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| Organization name |
MIT
|
| Department |
Koch Institute
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| Lab |
Bioinformatics Core
|
| Street address |
77 Massachusetts Ave
|
| City |
Cambridge |
| State/province |
Ma |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (157)
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| Relations |
| BioProject |
PRJNA727854 |
| SRA |
SRP318796 |
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