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Series GSE174012 Query DataSets for GSE174012
Status Public on Jun 06, 2023
Title Bulk RNA sequencing from sorted thymic populations and tumors from control Cdc20(+/D) and aneuploid Cdc20(D/AAA)mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goals of this study are to compare the transcriptome profile of specific thymic populations in control Cdc20(+/D) and Cdc20(D/AAA)mice after the induction of chromosome missegregation as well the profile of tumors generated in those mice.
Methods: Total RNA was isolated from primary cells or tumors, using the RNeasy Plus Mini Kit (Qiagen) and sequenced on an Illumina HiSeq2000. At least 5000 cells were sorted directly in lysis buffer for specifc T cell progenitor populations in the thymus while at least 5 million cells have used for RNA isolation form thymic lymphomas. Unless otherwise indicated samples from tumors were isolated without sorting specific polulations.
Overall design bulk RNA-seq of tumor and non-tumor thymus of control Cdc20(+/D) and Cdc20(D/AAA) mice.
Contributor(s) Trakala M, Amon A
Citation(s) 34266888
Submission date May 06, 2021
Last update date Jun 06, 2023
Contact name Dunaduan Ma
Organization name MIT
Department Koch Institute
Lab Bioinformatics Core
Street address 77 Massachusetts Ave
City Cambridge
State/province Ma
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (157)
GSM5284458 D18-182101
GSM5284459 D18-182102
GSM5284460 D18-182103
BioProject PRJNA727854
SRA SRP318796

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174012_RNASeq180717_gene_counts.txt.gz 2.4 Mb (ftp)(http) TXT
GSE174012_RNASeq200227_gene_counts.txt.gz 2.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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