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| Status |
Public on May 24, 2022 |
| Title |
Identification of dysregulated transcripts in nkx2.2(RNAi) planarians. |
| Organism |
Schmidtea mediterranea |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Nkx2.2 is an intestine-enriched transcription factor required for regeneration in planarians. The goal of this study was to identify differentially expressed transcripts in uninjured nkx2.2(RNAi) planarians.
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| Overall design |
Total RNA was isolated from uninjured egfp(RNAi) (control) and nkx2.2(RNAi) planarians (fed one time, three biological replicates each). Library generation (IIllumina TruSeq Stranded mRNA Prep Kit) and RNA sequencing (2x150bp paired-end, Illumina NovaSeq 6000) were conducted by the OMRF Clinical Genomics Core. Bioinformatic analysis was conducted using BBDuk, Bowtie2, and edgeR.
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| Contributor(s) |
Wong LL, Bruxvoort CG, Cejda NI, Delaney MR, Rodriguez Otero J, Forsthoefel DJ |
| Citation(s) |
35778403 |
| |
| Submission date |
May 11, 2021 |
| Last update date |
Jul 14, 2022 |
| Contact name |
David J Forsthoefel |
| E-mail(s) |
david-forsthoefel@omrf.org
|
| Phone |
405-271-4047
|
| Organization name |
Oklahoma Medical Research Foundation
|
| Department |
Genes and Human Disease Research Program
|
| Lab |
S-314
|
| Street address |
825 NE 13th St
|
| City |
Oklahoma |
| State/province |
OK |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL29967 |
Illumina NovaSeq 6000 (Schmidtea mediterranea) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE174246 |
Identification of dysregulated transcripts in planarians |
|
| Relations |
| BioProject |
PRJNA728884 |
| SRA |
SRP319347 |
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