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Status |
Public on Jan 01, 2015 |
Title |
Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen |
Organism |
Mycobacterium tuberculosis H37Rv |
Experiment type |
Expression profiling by array
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Summary |
Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen.
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Overall design |
Microarray experiments were performed as dual-color hybridizations on reporter level. In order to compensate specific effects of the dyes and to ensure statistically relevant data analysis, a color-swap dye-reversal was performed. Two different labeling methods were applied. RNA labeling was performed with the two color Quick Amp Labeling Kit (Agilent Technologies) using FullSpectrum MultiStart Primer for T7 IVT RNA Amplification (BioCat GmbH, Heidelberg, Germany) as random T7 labeling. Alternatively, the total RNA samples were amplified with the TransPlex Whole Transcriptome Amplification Kit (Sigma-Aldrich, Munich, Germany) and labeled with BioPrime Plus Array CGH Indirect Genomic Labeling System (Invitrogen, Karlsruhe, Germany) as WTA BioPrime indirect. Both methods were combined in the data analysis and only results derived from both labeling methods were considered as relevant for further verification.
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Contributor(s) |
Mollenkopf H, Kaufmann S, Warrier T |
Citation missing |
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Submission date |
Jul 30, 2009 |
Last update date |
Jan 01, 2015 |
Contact name |
Hans-Joachim Mollenkopf |
E-mail(s) |
mollenkopf@mpiib-berlin.mpg.de
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Phone |
+49 30 28460 482
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Organization name |
Max-Planck-Institute for Infection Biology
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Lab |
Microarray/Genomics Core Facility
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Street address |
Charitéplatz 1
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City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
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Platforms (1) |
GPL8951 |
Mycobacterium tuberculosis H37Rv custom 44K array |
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Samples (8)
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GSM434759 |
H37Rv 48h 50uM vs. H37Rv 48h control FS T7 |
GSM434760 |
H37Rv 48h control vs. H37Rv 48h 50uM FS T7 |
GSM434761 |
H37Rv 48h 100uM vs. H37Rv 48h control FS T7 |
GSM434763 |
H37Rv 48h control vs. H37Rv 48h 100uM FS T7 |
GSM434764 |
H37Rv 48h 50uM vs. H37Rv 48h control WTA Klenow indirect |
GSM434765 |
H37Rv 48h control vs. H37Rv 48h 50uM WTA Klenow indirect |
GSM434766 |
H37Rv 48h 100uM vs. H37Rv 48h control WTA Bioprime indirect |
GSM434767 |
H37Rv 48h control vs. H37Rv 48h 100uM WTA Bioprime indirect |
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Relations |
BioProject |
PRJNA118911 |