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Series GSE17424 Query DataSets for GSE17424
Status Public on Jan 01, 2015
Title Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen
Organism Mycobacterium tuberculosis H37Rv
Experiment type Expression profiling by array
Summary Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen.
 
Overall design Microarray experiments were performed as dual-color hybridizations on reporter level. In order to compensate specific effects of the dyes and to ensure statistically relevant data analysis, a color-swap dye-reversal was performed. Two different labeling methods were applied. RNA labeling was performed with the two color Quick Amp Labeling Kit (Agilent Technologies) using FullSpectrum MultiStart Primer for T7 IVT RNA Amplification (BioCat GmbH, Heidelberg, Germany) as random T7 labeling. Alternatively, the total RNA samples were amplified with the TransPlex Whole Transcriptome Amplification Kit (Sigma-Aldrich, Munich, Germany) and labeled with BioPrime Plus Array CGH Indirect Genomic Labeling System (Invitrogen, Karlsruhe, Germany) as WTA BioPrime indirect. Both methods were combined in the data analysis and only results derived from both labeling methods were considered as relevant for further verification.
 
Contributor(s) Mollenkopf H, Kaufmann S, Warrier T
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Submission date Jul 30, 2009
Last update date Jan 01, 2015
Contact name Hans-Joachim Mollenkopf
E-mail(s) mollenkopf@mpiib-berlin.mpg.de
Phone +49 30 28460 482
Organization name Max-Planck-Institute for Infection Biology
Lab Microarray/Genomics Core Facility
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platforms (1)
GPL8951 Mycobacterium tuberculosis H37Rv custom 44K array
Samples (8)
GSM434759 H37Rv 48h 50uM vs. H37Rv 48h control FS T7
GSM434760 H37Rv 48h control vs. H37Rv 48h 50uM FS T7
GSM434761 H37Rv 48h 100uM vs. H37Rv 48h control FS T7
Relations
BioProject PRJNA118911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
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Supplementary data files not provided
Processed data included within Sample table

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