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Series GSE174250 Query DataSets for GSE174250
Status Public on Dec 10, 2021
Title Cut&Run in Drosophila melanogaster control, mutant and knockdown ovaries
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary H3K27me3 profiles using Cleavage under targets and Release using nuclease (Cut&Run) in control and KD Drosophila melanogaster ovaries. We examined the impact on chromatin profiles in Drosophila melanogaster ovaries in which the lid, the Sin3a, the Snr1 or the mod(mdg4) gene have been selectively knocked down by tissue-specific shRNA expression. We additionally explored H3K27me3 and H3K9me3 in control and dhd mutant ovaries either carrying or not a transgene.
 
Overall design Cut&Run was used to characterize either H3K27me3 or H3K9me3 profile in control, knockdown or mutant Drosophila melanogaster ovaries in duplicates or triplicates.
 
Contributor(s) Orsi GA, Torres-Campana D, Loppin B
Citation(s) 34982772
Submission date May 11, 2021
Last update date Mar 14, 2022
Contact name Guillermo A Orsi
E-mail(s) guillermo.orsi@ens-lyon.fr
Organization name ENS Lyon/ LBMC-UMR 5239
Street address 46 allée d'Italie
City Lyon
ZIP/Postal code 69364
Country France
 
Platforms (1)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
Samples (28)
GSM5289848 Ctrl_K27me3_Ov_rep1
GSM5289849 Ctrl_K27me3_Ov_rep2
GSM5289850 Ctrl_K27me3_Ov_rep3
Relations
BioProject PRJNA728960
SRA SRP319468

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174250_RAW.tar 1.0 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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