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| Status |
Public on Dec 10, 2021 |
| Title |
Cut&Run in Drosophila melanogaster control, mutant and knockdown ovaries |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
H3K27me3 profiles using Cleavage under targets and Release using nuclease (Cut&Run) in control and KD Drosophila melanogaster ovaries. We examined the impact on chromatin profiles in Drosophila melanogaster ovaries in which the lid, the Sin3a, the Snr1 or the mod(mdg4) gene have been selectively knocked down by tissue-specific shRNA expression. We additionally explored H3K27me3 and H3K9me3 in control and dhd mutant ovaries either carrying or not a transgene.
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| Overall design |
Cut&Run was used to characterize either H3K27me3 or H3K9me3 profile in control, knockdown or mutant Drosophila melanogaster ovaries in duplicates or triplicates.
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| Contributor(s) |
Orsi GA, Torres-Campana D, Loppin B |
| Citation(s) |
34982772 |
| |
| Submission date |
May 11, 2021 |
| Last update date |
Mar 14, 2022 |
| Contact name |
Guillermo A Orsi |
| E-mail(s) |
guillermo.orsi@ens-lyon.fr
|
| Organization name |
ENS Lyon/ LBMC-UMR 5239
|
| Street address |
46 allée d'Italie
|
| City |
Lyon |
| ZIP/Postal code |
69364 |
| Country |
France |
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| Platforms (1) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA728960 |
| SRA |
SRP319468 |
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