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| Status |
Public on Oct 12, 2022 |
| Title |
Identification of Tcf21 downstream genes in the epicardial cells and cardiomyocytes by transcriptomic analysis |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Studying the epicardium-myocardium crosstalk in the zebrafish larval heart. To do so, we aimed to identify, with RNA-seq, the genes dysregulated following the loss of the epicardial marker gene tcf21 in sorted epicardial cells and cardiomyocytes.
Results: We first analyzed the transcriptome of epicardial and myocardial WT cells and identified cell-type specific/enriched genes. Then, we identified several differential expressed genes in tcf21 mutants, including several ligand-receptor couples known to mediate the epicardium-myocardium crosstalk.
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| Overall design |
Hearts were manually extracted from 96 hpf Tg(myl7:mcherry), TgBAC(tcf21:nls-EGFP) tcf21+/+ and tcf21-/- zebrafish larvae. Hearts were dissociated into single-cell suspensions and FACS was performed to sort epicardial and myocardial cells based on the fluorescence signal.
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| Contributor(s) |
Boezio GL, Guenther S |
| Citation(s) |
36172839 |
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| Submission date |
May 16, 2021 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Stefan Günther |
| E-mail(s) |
stefan.guenther@mpi-bn.mpg.de
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| Organization name |
MPI for heart and lung research
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| Street address |
ludwigtr. 43
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| City |
bad nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
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| Platforms (1) |
| GPL20828 |
Illumina NextSeq 500 (Danio rerio) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA730305 |
| SRA |
SRP319994 |
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