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| Status |
Public on Mar 31, 2010 |
| Title |
Detection of aberrant DNA methylation in AML cell lines compared to normal human monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
To globally define methylation-’prone’ and -’protected’ CpG islands in leukemia, we analyzed the methylation status of 23,000 CpG islands of the human genome in two acute leukemia cell lines as well as normal blood monocytes using our previously described methyl-CpG immunoprecipitation (MCIp) technique (Gebhard et al. 2006; Schilling and Rehli 2007). This method enriches for highly CpG methylated DNA that can be directly applied to fluorescent labeling and oligonucleotide microarray hybridization without an additional amplification step.
Keywords: MCIp-on-Chip; comparative genomic hybridization
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| Overall design |
CpG-methylated genomic DNA was enriched using methyl-CpG immunoprecipitation (MCIp). On each microarray, the enriched material from leukemia cell lines was compared to the enriched material from normal blood monocytes to identify aberrantly methylated regions. Three biological replicates were analysed for each cell line.
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| |
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| Contributor(s) |
Gebhard C, Rehli M |
| Citation(s) |
20145141 |
| |
| Submission date |
Jul 31, 2009 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Michael Rehli |
| E-mail(s) |
michael.rehli@klinik.uni-r.de
|
| Organization name |
University Hospital Regensburg
|
| Department |
Internal Med III
|
| Street address |
F.-J.-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
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| Platforms (1) |
| GPL8544 |
Human CpG Island Microarray 244K (Agilent-014791) with extended custom annotation |
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| Samples (6)
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| GSM435294 |
Comparative MCIp-on-Chip for U937 and human blood monocytes; Replicate 1 |
| GSM435295 |
Comparative MCIp-on-Chip for U937 and human blood monocytes; Replicate 2 |
| GSM435296 |
Comparative MCIp-on-Chip for U937 and human blood monocytes; Replicate 3 |
| GSM435297 |
Comparative MCIp-on-Chip for THP1 and human blood monocytes; Replicate 1 |
| GSM435298 |
Comparative MCIp-on-Chip for THP1 and human blood monocytes; Replicate 2 |
| GSM435299 |
Comparative MCIp-on-Chip for THP1 and human blood monocytes; Replicate 3 |
|
| Relations |
| BioProject |
PRJNA118727 |
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