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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jul 26, 2021 |
Title |
Systematic investigation of cytokine signaling activity at the tissue and single-cell levels |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: The role of different TGF-beta isoforms in tumor progression is not well established. The goal of the study was to assess the effect on the 4T1 mouse mammary tumor transcriptomes of treatment with isoform-selective anti-TGFbeta neutralizing antibodies to address whether sparing TGF-beta3 had any biological impact. Methods: 40,000 4T1 cells were implanted in the #4 mammary fatpad of syngeneic female BALB/c mice. Mice were treated with therapeutic or control antibodies (5mg/Kg i.p. 3x q wk) for 12 days prior to tumor harvest for RNA-Seq analysis. Treatments were: 1D11 pan-TGFbeta mouse monoclonal, XPA.42.681 pan-TGFbeta human monoclonal, XPA.42.089 anti-TGFbeta1+2 human monoclonal, anti-KLH control human monoclonal, 13C4 control mouse monoclonal, or vehicle control. Tumor mRNA profiles were generated by deep-sequencing of n=5 to 7 samples/treatment group using Illumina HiSeq2500 with TruSeq v4 chemistry. Results: 51-115 million pass-filtered reads were obtained for all samples, with >=91% of bases having a quality score of 30 or above.
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Overall design |
mRNA profiles of 4T1 mouse mammary tumors treated with isoform-selective anti-TGFbeta antibodies were generated by deep sequencing of n=5-7 samples/treatment group by Illumina HiSeq2500
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Contributor(s) |
Jiang P, Zhang Y, Ru B, Yang Y, Vu T, Paul R, Mirza A, Altan-Bonnet G, Liu L, Ruppin E, Wakefield L, Wucherpfennig KW |
Citation(s) |
34594031 |
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Submission date |
May 19, 2021 |
Last update date |
Oct 25, 2021 |
Contact name |
Maxwell Lee |
E-mail(s) |
leemax@mail.nih.gov
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Phone |
240-276-5239
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Organization name |
National Cancer Institute, NIH
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Department |
CCR
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Lab |
LCBG
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Street address |
Building 37, Room 5120
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City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (39)
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Relations |
BioProject |
PRJNA731117 |
SRA |
SRP320488 |
Supplementary file |
Size |
Download |
File type/resource |
GSE174686_LW_RNASeq39.anno_rpkm.txt.gz |
5.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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