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Series GSE174686 Query DataSets for GSE174686
Status Public on Jul 26, 2021
Title Systematic investigation of cytokine signaling activity at the tissue and single-cell levels
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The role of different TGF-beta isoforms in tumor progression is not well established. The goal of the study was to assess the effect on the 4T1 mouse mammary tumor transcriptomes of treatment with isoform-selective anti-TGFbeta neutralizing antibodies to address whether sparing TGF-beta3 had any biological impact.
Methods: 40,000 4T1 cells were implanted in the #4 mammary fatpad of syngeneic female BALB/c mice. Mice were treated with therapeutic or control antibodies (5mg/Kg i.p. 3x q wk) for 12 days prior to tumor harvest for RNA-Seq analysis. Treatments were: 1D11 pan-TGFbeta mouse monoclonal, XPA.42.681 pan-TGFbeta human monoclonal, XPA.42.089 anti-TGFbeta1+2 human monoclonal, anti-KLH control human monoclonal, 13C4 control mouse monoclonal, or vehicle control. Tumor mRNA profiles were generated by deep-sequencing of n=5 to 7 samples/treatment group using Illumina HiSeq2500 with TruSeq v4 chemistry.
Results: 51-115 million pass-filtered reads were obtained for all samples, with >=91% of bases having a quality score of 30 or above.
 
Overall design mRNA profiles of 4T1 mouse mammary tumors treated with isoform-selective anti-TGFbeta antibodies were generated by deep sequencing of n=5-7 samples/treatment group by Illumina HiSeq2500
 
Contributor(s) Jiang P, Zhang Y, Ru B, Yang Y, Vu T, Paul R, Mirza A, Altan-Bonnet G, Liu L, Ruppin E, Wakefield L, Wucherpfennig KW
Citation(s) 34594031
Submission date May 19, 2021
Last update date Oct 25, 2021
Contact name Maxwell Lee
E-mail(s) leemax@mail.nih.gov
Phone 240-276-5239
Organization name National Cancer Institute, NIH
Department CCR
Lab LCBG
Street address Building 37, Room 5120
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (39)
GSM5322491 LW_1
GSM5322492 LW_2
GSM5322493 LW_3
Relations
BioProject PRJNA731117
SRA SRP320488

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174686_LW_RNASeq39.anno_rpkm.txt.gz 5.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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