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| Status |
Public on Oct 31, 2021 |
| Title |
MicroRNA profiling of psoriatic skin identifies 11 miRNAs associated with disease severity |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as central regulators of gene expression and powerful biomarkers of disease. Much is yet unknown about their role in psoriasis pathology. To globally characterize the miRNAome of psoriatic skin, skin biopsies were collected from psoriatic cases (n = 75) and non-psoriatic controls (n = 57) and RNA sequenced. Count data was meta-analyzed with a previously published dataset (cases, n = 24, controls, n = 20), increasing the number of psoriatic cases four-fold from previously published studies. Differential gene expression analyses were performed comparing lesional psoriatic (PP), non-lesional psoriatic (PN) and control (NN) skin. Further, functional enrichment and cell-specific analyses were performed. Across all contrasts, we identified 439 significantly differentially expressed miRNAs (DEMs), of which 127 were novel for psoriasis and 11 were related to disease severity. Meta-analyses identified 20 DEMs between PN and NN, suggesting an inherent change in the constitution of all skin in psoriasis. By integrating the miRNA transcriptome with mRNA target interactions, we identified several functionally enriched terms, including ‘thyroid hormone signaling’, ‘insulin resistance’ and various infectious diseases. Cell specific expression analyses revealed that the upregulated DEMs were enriched in epithelial and immune cells. This study provides the most comprehensive overview of the miRNAome in psoriatic skin to date and identifies a miRNA signature related to psoriasis severity. Our results may represent molecular links between psoriasis and related comorbidities and have outlined potential directions for future functional studies to identify biomarkers and treatment targets.
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| Overall design |
Differential gene expression analyses were performed comparing lesional psoriatic (PP), non-lesional psoriatic (PN) and control (NN) skin.
NOTE from submitter: It is sensitive human sequencing data and the raw data can not be provided due to patient privacy concerns.
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| Contributor(s) |
Solvin ÅO, Chawla K, Olsen LC, Danielsen K, Jenssen M, Furberg A, Saunes M, Hveem K, Sætrom P, Løset M |
| Citation(s) |
34748247 |
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| Submission date |
May 20, 2021 |
| Last update date |
Feb 15, 2022 |
| Contact name |
Mari Løset |
| E-mail(s) |
mari.loset@ntnu.no
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| Phone |
+4790799117
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| Organization name |
NTNU
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| Department |
Department of Public Health and Nursing
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| Street address |
Håkon Jarls gate 11
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| City |
Trondheim |
| State/province |
Sør-trøndelag |
| ZIP/Postal code |
7491 |
| Country |
Norway |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (192)
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| Relations |
| BioProject |
PRJNA731486 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174763_normalized_miRNA_exp_matrix.txt.gz |
319.6 Kb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
| Raw data not provided for this record |
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