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| Status |
Public on May 22, 2021 |
| Title |
Common features of aging fail to occur in Drosophila raised without a bacterial microbiome |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
By comparing the transcriptomes of Drosophila raised either under conventional conditions or in the complete absence of bacteria, we find that 70% of the transcriptional changes that normally occur with age are actually adaptive responses to the microbiome, not unavoidable features of aging. In the germ-free state, these seemingly ‘age-dependent’ gene expression changes never happen.
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| Overall design |
Total RNA was isolated from the heads of wild type Drosophila grown either under conventional conditions (3, 10, 30 and 45 days old) or in the presence of antibiotics (10, 30 and 45 days old). Genome-wide gene expression was assayed using Affymetrix DroGene 1.0 ST microarrays, and data was analyzed as described in the associated manusript.
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| Contributor(s) |
Giniger E, Johnson KR |
| Citation(s) |
34235409 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| ZIA NS003106 |
Roles of Cdk5 in neurodevelopment and neurodegeneration |
National Institute of Neurological Disorders and Stroke |
Edward Giniger |
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| |
| Submission date |
May 21, 2021 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Edward Giniger |
| E-mail(s) |
ginigere@ninds.nih.gov
|
| Phone |
301-451-3890
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| Organization name |
NIH
|
| Department |
NINDS
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| Street address |
Bldg 35,Rm 1C-1002; 35 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL18094 |
[DroGene-1_0-st] Drosophila Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA731913 |
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