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Series GSE17535 Query DataSets for GSE17535
Status Public on Aug 03, 2010
Title Developmental time-course study of Drosophila melanogaster, D. sechellia, D. simulans, and D. sim x D.s sec hybrids
Platform organism Drosophila melanogaster
Sample organisms Drosophila melanogaster; Drosophila sechellia; Drosophila simulans; Drosophila sechellia x Drosophila simulans
Experiment type Expression profiling by array
Summary In order to test the hypothesis that adult hybrid misexpression results from the cascading effect of earlier-expressed developmentally important improperly regulated genes, as well as address whether Von Baer’s 3rd law (suggesting that earlier stages of development should be more similar between species than later stages) holds at the level of gene expression, we conducted whole-transcriptome Drosophila melanogaster cDNA microarray-based expression profiling of males of D. melanogaster, D. sechellia, and D. simulans, at four synchronized developmental time-points (3rd instar larval [larval], early pupal, late pupal, and newly-emerged adult [adult]). D. simulans and D. sechellia shared a most recent common ancestor (MRCA) ~0.5 to 1.0 million years ago (MYA) and form a clade that shared an MRCA with D. melanogaster approximately 5.4 MYA. In addition, we also performed the same analysis on the male interspecific F1 hybrids of the D. simulans (♀) × D. sechellia (♂) cross.
 
Overall design Males from each species and hybrids were synchronized within a 2h window (1.5h for adults) at the beginning of the stage during which they were collected (larval, early pupal, late pupal and adult) and 25 staged individuals were collected and their RNA extracted and amplified twice according to the protocols associated with each array. For the three pure species, 3 technical replicates were performed for each stage; however, in the case of the D. simulans x D. sechellia F1 hybrids, three separate extractions of 25 individuals were performed at each stage, such that they acted as biological as well as technical replicates. Arrays were scanned and analyzed as indicated in the descriptions of each array with the intent of profiling how gene expression changes over development within species as well as comparing how expression levels evolve during development between species. The hybrids were used in order to analyze how mechanisms of gene regulation diverge between species as manifested as misexpression of genes in the hybrids relative to their parents.
 
Contributor(s) Artieri CG, Singh RS
Citation(s) 20356354
Submission date Aug 06, 2009
Last update date Jan 18, 2013
Contact name Rama S. Singh
E-mail(s) singh@mcmaster.ca
Organization name McMaster University
Department Biology Department
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S 4K1
Country Canada
 
Platforms (1)
GPL3598 CDMC_Drosophila_12k2
Samples (48)
GSM435987 D. melanogaster male larval replicate 1
GSM436078 D. melanogaster male larval replicate 2
GSM436079 D. melanogaster male larval replicate 3
Relations
BioProject PRJNA118643

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Supplementary data files not provided
Processed data included within Sample table

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