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| Status |
Public on Jul 15, 2021 |
| Title |
Drosophila architectural protein CTCF is not essential for fly survival and is able to function independently of CP190 |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
CTCF is the most likely ancestor of proteins that contain large clusters of C2H2 zinc finger domains (C2H2) and is conserved among most bilateral organisms. In mammals, CTCF functions as the main architectural protein involved in the organization of topology-associated domains (TADs). In vertebrates and Drosophila, CTCF is involved in the regulation of homeotic genes. Previously, null mutations in the dCTCF gene were found to result in death during the stage of pharate adults, which failed to eclose from their pupal case. Here, we obtained several new null dCTCF mutations and found that the complete inactivation of dCTCF appears to be limited to phenotypic manifestations of the Abd-B gene and fertility of adult flies. Many modifiers that are not associated with an independent phenotypic manifestation can significantly enhance the expressivity of the null dCTCF mutations, indicating that other architectural proteins are able to functionally compensate for dCTCF inactivation in Drosophila. We also mapped the 715–735 aa region of dCTCF as being essential for the interaction with the BTB (Broad-Complex, Tramtrack, and Bric a brac) and microtubule-targeting (M) domains of the CP190 protein, which binds to many architectural proteins. However, the mutational analysis showed that the interaction with CP190 was not essential for the functional activity of dCTCF in vivo.
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| Overall design |
ChIP-seq signal of the HA, dCTCF and CP190 architectural/insulator proteins occupancy in whole adult flies in dCTCF wt and dCTCFΔ705-733 lines.
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| Contributor(s) |
Maksimenko O, Klimenko N |
| Citation(s) |
34311130 |
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| Submission date |
May 24, 2021 |
| Last update date |
Oct 15, 2021 |
| Contact name |
Natalia Klimenko |
| E-mail(s) |
lklimenko@genebiology.ru
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| Phone |
9150884603
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| Organization name |
Institute of Gene Biology (IGB) of the Russian Academy of Sciences
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| Street address |
34/5 Vavilova Street
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| City |
Moscow |
| ZIP/Postal code |
143026 |
| Country |
Russia |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA732305 |
| SRA |
SRP321085 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE175402_CP190_dCTCF_delta_100_RPKM.bedGraph.gz |
7.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE175402_CP190_dCTCF_delta_vs_PI_mouse.narrowPeak.gz |
160.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE175402_CP190_dCTCF_wt_100_RPKM.bedGraph.gz |
7.6 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE175402_CP190_dCTCF_wt_vs_PI_mouse.narrowPeak.gz |
127.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE175402_HA_dCTCF_delta_100_RPKM.bedGraph.gz |
6.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE175402_HA_dCTCF_delta_vs_PI_mouse.narrowPeak.gz |
34.7 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE175402_HA_dCTCF_wt_100_RPKM.bedGraph.gz |
7.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE175402_HA_dCTCF_wt_vs_PI_mouse.narrowPeak.gz |
33.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE175402_RAW.tar |
12.7 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
| GSE175402_dCTCF_dCTCF_delta_100_RPKM.bedGraph.gz |
7.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE175402_dCTCF_dCTCF_delta_vs_PI_rat.narrowPeak.gz |
35.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE175402_dCTCF_dCTCF_wt_100_RPKM.bedGraph.gz |
7.3 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE175402_dCTCF_dCTCF_wt_vs_PI_rat.narrowPeak.gz |
38.4 Kb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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